@article{8c4772ba522e45df9694de3baea131fc,
title = "Distinct sensitivities to SARS-CoV-2 variants in vaccinated humans and mice",
abstract = "The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 2019 has led to the development of a large number of vaccines, several of which are now approved for use in humans. Understanding vaccine-elicited antibody responses against emerging SARS-CoV-2 variants of concern (VOCs) in real time is key to inform public health policies. Serum neutralizing antibody titers are the current best correlate of protection from SARS-CoV-2 challenge in non-human primates and a key metric to understand immune evasion of VOCs. We report that vaccinated BALB/c mice do not recapitulate faithfully the breadth and potency of neutralizing antibody responses elicited by various vaccine platforms against VOCs, compared with non-human primates or humans, suggesting caution should be exercised when interpreting data obtained with this animal model.",
keywords = "antibodies, COVID-19, CP: Immunology, CP: Microbiology, humans, mouse, non-human primates, SARS-CoV-2, vaccines",
author = "Walls, {Alexandra C.} and VanBlargan, {Laura A.} and Kai Wu and Angela Choi and Navarro, {Mary Jane} and Diana Lee and Laura Avena and Berrueta, {Daniela Montes} and Pham, {Minh N.} and Sayda Elbashir and Kraft, {John C.} and Miranda, {Marcos C.} and Elizabeth Kepl and Max Johnson and Alyssa Blackstone and Kaitlin Sprouse and Brooke Fiala and O'Connor, {Megan A.} and Natalie Brunette and Arunachalam, {Prabhu S.} and Lisa Shirreff and Kenneth Rogers and Lauren Carter and Fuller, {Deborah H.} and Francois Villinger and Bali Pulendran and Diamond, {Michael S.} and Edwards, {Darin K.} and King, {Neil P.} and David Veesler",
note = "Funding Information: We thank Hideki Tani (University of Toyama) for providing the reagents necessary for preparing VSV pseudotyped viruses and Wesley Van Voorhis for coordination of human sera samples. The authors thank Kathryn Guerriero, Brieann Brown, Solomon Wangari, Joel Ahrens, Naoto Iwayama, and William Garrison for their technical assistance with the pigtail macaque study This study was supported by the National Institute of Allergy and Infectious Diseases (DP1AI158186 and HHSN272201700059C to D.V. R01 AI157155 to M.S.D. and Influenza Research and Response [CEIRR] contract 75N93021C00014 to M.S.D.), National Institute of Health Office of Research Infrastructure Programs (U42OD011123 and P51-OD010425, Washington National Primate Research Center), a Pew Biomedical Scholars Award (D.V.), an Investigators in the Pathogenesis of Infectious Disease Awards from the Burroughs Wellcome Fund (D.V.), Fast Grants (D.V.), and the Bill & Melinda Gates Foundation (OPP1156262 to N.P.K. and D.V.). D.V. is an Investigator of the Howard Hughes Medical Institute. A.C.W. and D.V. conceived the project and designed experiments. A.C.W. L.A.V. K.W. A.C. M.J.N. D.L. L.A. D.M.B. S.E. and K.S. performed experiments. M.N.P. J.C.K. M.C.M. E.K. M.J. A.B. B.F. M.A.O. N.B. P.S.A. L.S. K.R. L.C. D.H.F. F.V. B.P. and N.P.K. prepared immunogens and coordinated immunizations. A.C.W. D.H.F. M.S.D. D.K.E. N.P.K. and D.V. supervised the project and obtained funding. A.C.W. and D.V. analyzed the data and wrote the manuscript with input from all authors. A.C.W. N.P.K. and D.V. are named as inventors on patent applications filed by the University of Washington based on the RBD-NP presented in this paper. N.P.K. is a co-founder, shareholder, paid consultant, and chair of the scientific advisory board of Icosavax, Inc. and the King lab has received an unrelated sponsored research agreement from Pfizer. M.S.D. is a consultant for Inbios, Vir Biotechnology, and Carnival Corporation, and on the Scientific Advisory Boards of Moderna and Immunome. The Diamond laboratory has received unrelated funding support in sponsored research agreements from Vir Biotechnology, Kaleido, and Emergent BioSolutions and past support from Moderna not related to these studies. K.W. A.C. and D.K.E are employees of Moderna and hold stock/stock options in the company. D.H.F. has equity interest in HDT Bio. Funding Information: We thank Hideki Tani (University of Toyama) for providing the reagents necessary for preparing VSV pseudotyped viruses and Wesley Van Voorhis for coordination of human sera samples. The authors thank Kathryn Guerriero, Brieann Brown, Solomon Wangari, Joel Ahrens, Naoto Iwayama, and William Garrison for their technical assistance with the pigtail macaque study This study was supported by the National Institute of Allergy and Infectious Diseases ( DP1AI158186 and HHSN272201700059C to D.V., R01 AI157155 to M.S.D., and Influenza Research and Response [ CEIRR ] contract 75N93021C00014 to M.S.D. ), National Institute of Health Office of Research Infrastructure Programs (U42OD011123 and P51-OD010425, Washington National Primate Research Center), a Pew Biomedical Scholars Award (D.V.), an Investigators in the Pathogenesis of Infectious Disease Awards from the Burroughs Wellcome Fund (D.V.), Fast Grants (D.V.), and the Bill & Melinda Gates Foundation ( OPP1156262 to N.P.K. and D.V.). D.V. is an Investigator of the Howard Hughes Medical Institute. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = aug,
day = "30",
doi = "10.1016/j.celrep.2022.111299",
language = "English",
volume = "40",
journal = "Cell Reports",
issn = "2211-1247",
number = "9",
}