Dissociation of Placental Tissues for Single-Cell Techniques

Valeria Garcia-Flores, Derek Miller, Jose Galaz, Nardhy Gomez-Lopez

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


The dissociation of whole tissue into single-cell suspensions is a critical step for techniques focused on profiling of individual cells. Here, we describe a protocol for the preparation of high-quality single-cell suspensions from human placental tissues: the basal plate (BP), placental villi (PV), and chorioamniotic membranes (CAM). This protocol also provides guidance for the cryopreservation and recovery of single-cell suspensions for later use. The methods described here have been demonstrated to be suitable for downstream single-cell applications, such as single-cell RNA-sequencing, that require viable, high-quality cell suspensions.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages12
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Basal plate
  • Chorioamniotic membranes
  • Placental villi
  • Single-cell RNA-sequencing


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