@inbook{e04a37fec91d43eebb790da8c04e9f5d,
title = "Dissociation of Placental Tissues for Single-Cell Techniques",
abstract = "The dissociation of whole tissue into single-cell suspensions is a critical step for techniques focused on profiling of individual cells. Here, we describe a protocol for the preparation of high-quality single-cell suspensions from human placental tissues: the basal plate (BP), placental villi (PV), and chorioamniotic membranes (CAM). This protocol also provides guidance for the cryopreservation and recovery of single-cell suspensions for later use. The methods described here have been demonstrated to be suitable for downstream single-cell applications, such as single-cell RNA-sequencing, that require viable, high-quality cell suspensions.",
keywords = "Basal plate, Chorioamniotic membranes, Placental villi, Single-cell RNA-sequencing",
author = "Valeria Garcia-Flores and Derek Miller and Jose Galaz and Nardhy Gomez-Lopez",
note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3746-3_13",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "143--154",
booktitle = "Methods in Molecular Biology",
}