@article{d2b9eaeafd1b4225981edd695349421c,
title = "Disentangling glial diversity in peripheral nerves at single-nuclei resolution",
abstract = "The peripheral nerve contains diverse cell types that support its proper function and maintenance. In this study, we analyzed multiple peripheral nerves using single-nuclei RNA sequencing, which allowed us to circumvent difficulties encountered in analyzing cells with complex morphologies via conventional single-cell methods. The resultant mouse peripheral nerve cell atlas highlights a diversity of cell types, including multiple subtypes of Schwann cells (SCs), immune cells and stromal cells. We identified a distinct myelinating SC subtype that expresses Cldn14, Adamtsl1 and Pmp2 and preferentially ensheathes motor axons. The number of these motor-associated Pmp2+ SCs is reduced in both an amyotrophic lateral sclerosis (ALS) SOD1G93A mouse model and human ALS nerve samples. Our findings reveal the diversity of SCs and other cell types in peripheral nerve and serve as a reference for future studies of nerve biology and disease.",
author = "Yim, {Aldrin K.Y.} and Wang, {Peter L.} and John Bermingham and Amber Hackett and Amy Strickland and Timothy Miller and Cindy Ly and Mitra, {Robi D.} and Jeffrey Milbrandt",
note = "Funding Information: The authors thank all our collaborators at the Washington University School of Medicine for their advice and discussion. We thank R. Head, R. Barve and P. Cliften at GTAC@MGI and the McDonnell Genome Institute for discussion and assistance with RNA-seq. The authors are grateful for assistance from G. Randolph{\textquoteright}s laboratory and B. Zinselmeyer for confocal imaging and use of equipment (supported by National Institutes of Health (NIH) grants R01DK119147 and R37AI049653 to G.R.). The authors thank M. Shabsovich, T. Shen and C. Kreple for ALS SOD1 mice, M. Ireland for curating patient samples (supported by NIH grant R01NS078398 to T.M.) and R. Schmidt for curating clinical pathology reports and many helpful discussions. The authors thank R. McClarney and C. Menendez for experimental assistance and members of the Milbrandt and DiAntonio laboratories for helpful comments and discussion. This project was supported by NIH grants RF1MH117070 and RO1GM123203 to R.D.M. and R01NS105645 and R01AG013730 to J.M. G93A Funding Information: The authors thank all our collaborators at the Washington University School of Medicine for their advice and discussion. We thank R. Head, R. Barve and P. Cliften at GTAC@MGI and the McDonnell Genome Institute for discussion and assistance with RNA-seq. The authors are grateful for assistance from G. Randolph?s laboratory and B. Zinselmeyer for confocal imaging and use of equipment (supported by National Institutes of Health (NIH) grants R01DK119147 and R37AI049653 to G.R.). The authors thank M. Shabsovich, T. Shen and C. Kreple for ALS SOD1G93A mice, M. Ireland for curating patient samples (supported by NIH grant R01NS078398 to T.M.) and R. Schmidt for curating clinical pathology reports and many helpful discussions. The authors thank R. McClarney and C. Menendez for experimental assistance and members of the Milbrandt and DiAntonio laboratories for helpful comments and discussion. This project was supported by NIH grants RF1MH117070 and RO1GM123203 to R.D.M. and R01NS105645 and R01AG013730 to J.M. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2022",
month = feb,
doi = "10.1038/s41593-021-01005-1",
language = "English",
volume = "25",
pages = "238--251",
journal = "Nature Neuroscience",
issn = "1097-6256",
number = "2",
}