Disease-specific alterations in the enteric virome in inflammatory bowel disease

Jason M. Norman; Scott A. Handley; Megan T. Baldridge; Lindsay Droit; Catherine Y. Liu; Brian C. Keller; Amal Kambal; Cynthia L. Monaco; Guoyan Zhao; Phillip Fleshner; Thaddeus Stappenbeck; Dermot P.B. McGovern; Ali Keshavarzian; Ece A. Mutlu; Jenny Sauk; Dirk Gevers; Ramnik J. Xavier; David Wang; Miles Parkes; Skip Virgin

doi:10.1016/j.cell.2015.01.002

Disease-specific alterations in the enteric virome in inflammatory bowel disease

Jason M. Norman, Scott A. Handley, Megan T. Baldridge, Lindsay Droit, Catherine Y. Liu, Brian C. Keller, Amal Kambal, Cynthia L. Monaco, Guoyan Zhao, Phillip Fleshner, Thaddeus Stappenbeck, Dermot P.B. McGovern, Ali Keshavarzian, Ece A. Mutlu, Jenny Sauk, Dirk Gevers, Ramnik J. Xavier, David Wang, Miles Parkes, Skip Virgin

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903 Scopus citations

Abstract

Summary Decreases in the diversity of enteric bacterial populations are observed in patients with Crohn's disease (CD) and ulcerative colitis (UC). Less is known about the virome in these diseases. We show that the enteric virome is abnormal in CD and UC patients. In-depth analysis of preparations enriched for free virions in the intestine revealed that CD and UC were associated with a significant expansion of Caudovirales bacteriophages. The viromes of CD and UC patients were disease and cohort specific. Importantly, it did not appear that expansion and diversification of the enteric virome was secondary to changes in bacterial populations. These data support a model in which changes in the virome may contribute to intestinal inflammation and bacterial dysbiosis. We conclude that the virome is a candidate for contributing to, or being a biomarker for, human inflammatory bowel disease and speculate that the enteric virome may play a role in other diseases.

Original language	English
Pages (from-to)	447-460
Number of pages	14
Journal	Cell
Volume	160
Issue number	3
DOIs	https://doi.org/10.1016/j.cell.2015.01.002
State	Published - Jan 29 2015

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Norman, J. M., Handley, S. A., Baldridge, M. T., Droit, L., Liu, C. Y., Keller, B. C., Kambal, A., Monaco, C. L., Zhao, G., Fleshner, P., Stappenbeck, T., McGovern, D. P. B., Keshavarzian, A., Mutlu, E. A., Sauk, J., Gevers, D., Xavier, R. J., Wang, D., Parkes, M., & Virgin, S. (2015). Disease-specific alterations in the enteric virome in inflammatory bowel disease. Cell, 160(3), 447-460. https://doi.org/10.1016/j.cell.2015.01.002

@article{9fbf8049747545f68735e8b69ac44b6b,

title = "Disease-specific alterations in the enteric virome in inflammatory bowel disease",

abstract = "Summary Decreases in the diversity of enteric bacterial populations are observed in patients with Crohn's disease (CD) and ulcerative colitis (UC). Less is known about the virome in these diseases. We show that the enteric virome is abnormal in CD and UC patients. In-depth analysis of preparations enriched for free virions in the intestine revealed that CD and UC were associated with a significant expansion of Caudovirales bacteriophages. The viromes of CD and UC patients were disease and cohort specific. Importantly, it did not appear that expansion and diversification of the enteric virome was secondary to changes in bacterial populations. These data support a model in which changes in the virome may contribute to intestinal inflammation and bacterial dysbiosis. We conclude that the virome is a candidate for contributing to, or being a biomarker for, human inflammatory bowel disease and speculate that the enteric virome may play a role in other diseases.",

author = "Norman, {Jason M.} and Handley, {Scott A.} and Baldridge, {Megan T.} and Lindsay Droit and Liu, {Catherine Y.} and Keller, {Brian C.} and Amal Kambal and Monaco, {Cynthia L.} and Guoyan Zhao and Phillip Fleshner and Thaddeus Stappenbeck and McGovern, {Dermot P.B.} and Ali Keshavarzian and Mutlu, {Ece A.} and Jenny Sauk and Dirk Gevers and Xavier, {Ramnik J.} and David Wang and Miles Parkes and Skip Virgin",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",

year = "2015",

month = jan,

day = "29",

doi = "10.1016/j.cell.2015.01.002",

language = "English",

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Norman, JM, Handley, SA , Baldridge, MT, Droit, L, Liu, CY, Keller, BC, Kambal, A, Monaco, CL, Zhao, G, Fleshner, P, Stappenbeck, T, McGovern, DPB, Keshavarzian, A, Mutlu, EA, Sauk, J, Gevers, D, Xavier, RJ, Wang, D, Parkes, M & Virgin, S 2015, 'Disease-specific alterations in the enteric virome in inflammatory bowel disease', Cell, vol. 160, no. 3, pp. 447-460. https://doi.org/10.1016/j.cell.2015.01.002

TY - JOUR

T1 - Disease-specific alterations in the enteric virome in inflammatory bowel disease

AU - Norman, Jason M.

AU - Handley, Scott A.

AU - Baldridge, Megan T.

AU - Droit, Lindsay

AU - Liu, Catherine Y.

AU - Keller, Brian C.

AU - Kambal, Amal

AU - Monaco, Cynthia L.

AU - Zhao, Guoyan

AU - Fleshner, Phillip

AU - Stappenbeck, Thaddeus

AU - McGovern, Dermot P.B.

AU - Keshavarzian, Ali

AU - Mutlu, Ece A.

AU - Sauk, Jenny

AU - Gevers, Dirk

AU - Xavier, Ramnik J.

AU - Wang, David

AU - Parkes, Miles

AU - Virgin, Skip

PY - 2015/1/29

Y1 - 2015/1/29

N2 - Summary Decreases in the diversity of enteric bacterial populations are observed in patients with Crohn's disease (CD) and ulcerative colitis (UC). Less is known about the virome in these diseases. We show that the enteric virome is abnormal in CD and UC patients. In-depth analysis of preparations enriched for free virions in the intestine revealed that CD and UC were associated with a significant expansion of Caudovirales bacteriophages. The viromes of CD and UC patients were disease and cohort specific. Importantly, it did not appear that expansion and diversification of the enteric virome was secondary to changes in bacterial populations. These data support a model in which changes in the virome may contribute to intestinal inflammation and bacterial dysbiosis. We conclude that the virome is a candidate for contributing to, or being a biomarker for, human inflammatory bowel disease and speculate that the enteric virome may play a role in other diseases.

AB - Summary Decreases in the diversity of enteric bacterial populations are observed in patients with Crohn's disease (CD) and ulcerative colitis (UC). Less is known about the virome in these diseases. We show that the enteric virome is abnormal in CD and UC patients. In-depth analysis of preparations enriched for free virions in the intestine revealed that CD and UC were associated with a significant expansion of Caudovirales bacteriophages. The viromes of CD and UC patients were disease and cohort specific. Importantly, it did not appear that expansion and diversification of the enteric virome was secondary to changes in bacterial populations. These data support a model in which changes in the virome may contribute to intestinal inflammation and bacterial dysbiosis. We conclude that the virome is a candidate for contributing to, or being a biomarker for, human inflammatory bowel disease and speculate that the enteric virome may play a role in other diseases.

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U2 - 10.1016/j.cell.2015.01.002

DO - 10.1016/j.cell.2015.01.002

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AN - SCOPUS:84922245262

SN - 0092-8674

VL - 160

SP - 447

EP - 460

JO - Cell

JF - Cell

IS - 3

ER -

Disease-specific alterations in the enteric virome in inflammatory bowel disease

Abstract

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