TY - JOUR
T1 - Discovery and Identification of Three Homocysteine Metabolites by Chemical Derivatization and Mass Spectrometry Fragmentation
AU - Agongo, Julius
AU - Grady, Scott F.
AU - Cho, Kevin
AU - Patti, Gary J.
AU - Bythell, Benjamin J.
AU - Arnatt, Christopher K.
AU - Edwards, James L.
N1 - Publisher Copyright:
© 2024 American Chemical Society.
PY - 2024/7/23
Y1 - 2024/7/23
N2 - Discovery and identification of a new endogenous metabolite are typically hindered by requirements of large sample volumes and multistage purifications to guide synthesis of the standard. Presented here is a metabolomics platform that uses chemical tagging and tandem mass spectrometry to determine structure, direct synthesis, and confirm identity. Three new homocysteine metabolites are reported: N-succinyl homocysteine, 2-methyl-1,3-thiazinane-4-carboxylic acid (MTCA), and homolanthinone.
AB - Discovery and identification of a new endogenous metabolite are typically hindered by requirements of large sample volumes and multistage purifications to guide synthesis of the standard. Presented here is a metabolomics platform that uses chemical tagging and tandem mass spectrometry to determine structure, direct synthesis, and confirm identity. Three new homocysteine metabolites are reported: N-succinyl homocysteine, 2-methyl-1,3-thiazinane-4-carboxylic acid (MTCA), and homolanthinone.
UR - http://www.scopus.com/inward/record.url?scp=85198128469&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.4c01706
DO - 10.1021/acs.analchem.4c01706
M3 - Article
C2 - 38976774
AN - SCOPUS:85198128469
SN - 0003-2700
VL - 96
SP - 11639
EP - 11643
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 29
ER -