TY - JOUR
T1 - Disassociation of lysosomal enzyme secretion and macrophage-mediated bone resorption
AU - Blair, Harry
AU - Kahn, Arnold J.
AU - Sadow, Jenny
AU - Malone, J. David
AU - Teitelbaum, Steven L.
PY - 1981/6/16
Y1 - 1981/6/16
N2 - We studied secretion of three lysosomal enzymes, acid phosphatase, β-glucuronidase and N-acetyl-glucosaminidase, by macrophages after 96 hours of culture in the presence or absence of 10-6 M cortisol. The steroid enhances (P<.001) macrophage-mediated resorption of devitalized rat bone particles as measured by release of previously incorporated 45Ca. However, medium activity of each of the three enzymes is diminished by 10-6 M cortisol. These data demonstrate that bone resorption may be stimulated in the presence of suppressed lysosomal enzyme secretion and challenge the assumption that these enzymes play an essential role in the resorption process.
AB - We studied secretion of three lysosomal enzymes, acid phosphatase, β-glucuronidase and N-acetyl-glucosaminidase, by macrophages after 96 hours of culture in the presence or absence of 10-6 M cortisol. The steroid enhances (P<.001) macrophage-mediated resorption of devitalized rat bone particles as measured by release of previously incorporated 45Ca. However, medium activity of each of the three enzymes is diminished by 10-6 M cortisol. These data demonstrate that bone resorption may be stimulated in the presence of suppressed lysosomal enzyme secretion and challenge the assumption that these enzymes play an essential role in the resorption process.
UR - https://www.scopus.com/pages/publications/0019789916
U2 - 10.1016/0006-291X(81)91916-1
DO - 10.1016/0006-291X(81)91916-1
M3 - Article
C2 - 7271811
AN - SCOPUS:0019789916
SN - 0006-291X
VL - 100
SP - 959
EP - 964
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -