Disassociation of lysosomal enzyme secretion and macrophage-mediated bone resorption

Harry Blair, Arnold J. Kahn, Jenny Sadow, J. David Malone, Steven L. Teitelbaum

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We studied secretion of three lysosomal enzymes, acid phosphatase, β-glucuronidase and N-acetyl-glucosaminidase, by macrophages after 96 hours of culture in the presence or absence of 10-6 M cortisol. The steroid enhances (P<.001) macrophage-mediated resorption of devitalized rat bone particles as measured by release of previously incorporated 45Ca. However, medium activity of each of the three enzymes is diminished by 10-6 M cortisol. These data demonstrate that bone resorption may be stimulated in the presence of suppressed lysosomal enzyme secretion and challenge the assumption that these enzymes play an essential role in the resorption process.

Original languageEnglish
Pages (from-to)959-964
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume100
Issue number3
DOIs
StatePublished - Jun 16 1981

Fingerprint Dive into the research topics of 'Disassociation of lysosomal enzyme secretion and macrophage-mediated bone resorption'. Together they form a unique fingerprint.

  • Cite this