We studied secretion of three lysosomal enzymes, acid phosphatase, β-glucuronidase and N-acetyl-glucosaminidase, by macrophages after 96 hours of culture in the presence or absence of 10-6 M cortisol. The steroid enhances (P<.001) macrophage-mediated resorption of devitalized rat bone particles as measured by release of previously incorporated 45Ca. However, medium activity of each of the three enzymes is diminished by 10-6 M cortisol. These data demonstrate that bone resorption may be stimulated in the presence of suppressed lysosomal enzyme secretion and challenge the assumption that these enzymes play an essential role in the resorption process.
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jun 16 1981|