Direct presynaptic regulation of GABA/glycine release by kainate receptors in the dorsal horn: An ionotropic mechanism

Geoffrey A. Kerchner, Guo Du Wang, Chang Shen Qiu, James E. Huettner, Min Zhuo

Research output: Contribution to journalArticle

97 Scopus citations

Abstract

In the spinal cord dorsal horn, excitatory sensory fibers terminate adjacent to interneuron terminals. Here, we show that kainate (KA) receptor activation triggered action potential-independent release of GABA and glycine from dorsal horn interneurons. This release was transient, because KA receptors desensitized, and it required Na+ entry and Ca2+ channel activation. KA modulated evoked inhibitory transmission in a dose-dependent, biphasic manner, with suppression being more prominent. In recordings from isolated neuron pairs, this suppression required GABAB receptor activation, suggesting that KA-triggered GABA release activated presynaptic GABAB autoreceptors. Finally, glutamate released from sensory fibers caused a KA and GABAB receptor-dependent suppression of inhibitory transmission in spinal slices. Thus, we show how presynaptic KA receptors are linked to changes in GABA/glycine release and highlight a novel role for these receptors in regulating sensory transmission.

Original languageEnglish
Pages (from-to)477-488
Number of pages12
JournalNeuron
Volume32
Issue number3
DOIs
StatePublished - Nov 8 2001

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