Direct preparations from chorionic villi–relationship between villous morphology and mitotic index

A. Romagnano, T. Featherstone, L. Sun, J. P. Crane, S. W. Cheung

    Research output: Contribution to journalArticlepeer-review

    3 Scopus citations

    Abstract

    It has been postulated that chorionic villi with abundant sprouts have a higher mitotic index and are therefore preferable for obtaining direct chromosome preparations from chorionic villus samples. This theory was tested by correlating villous morphology with mitotic index. Surprisingly, no statistically significant relationship was found. Choice of culture medium, however, was found to be important, with serum‐free RPMI yielding a higher mitotic index than 40 per cent FCS in MEM. We conclude that villous morphology, as assessed in this study, is not a major factor in determining the success of direct chromosome preparations.

    Original languageEnglish
    Pages (from-to)385-391
    Number of pages7
    JournalPrenatal Diagnosis
    Volume9
    Issue number6
    DOIs
    StatePublished - Jun 1989

    Keywords

    • Chorionic
    • Direct
    • Mitotic index
    • Prenatal
    • Villous morphology
    • chromosome
    • diagnosis
    • preparation
    • sampling
    • villus

    Fingerprint

    Dive into the research topics of 'Direct preparations from chorionic villi–relationship between villous morphology and mitotic index'. Together they form a unique fingerprint.

    Cite this