Direct electrochemistry of cytochrome P450 27B1 in surfactant films

Steve Y. Rhieu; Daniel R. Ludwig; Vince S. Siu; G. Tayhas R. Palmore

doi:10.1016/j.elecom.2009.08.002

Direct electrochemistry of cytochrome P450 27B1 in surfactant films

Steve Y. Rhieu, Daniel R. Ludwig, Vince S. Siu, G. Tayhas R. Palmore

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We report the first direct electrochemistry of cytochrome P450 27B1 (CYP27B1) immobilized on an edge-plane pyrolytic graphite (EPG) electrode coated with a film of didodecyldimethylammonium bromide (DDAB). Cyclic voltammetry (CV) in a deoxygenated solution revealed excellent electrochemical reversibility with an average midpoint potential of -180 ± 5 mV vs. Ag/AgCl and an apparent surface coverage of (7.0 ± 2.5) × 10¹³ molecules per cm². The rate of heterogeneous electron transfer between CYP27B1 and the EPG electrode was determined to be 3.5 ± 0.6 s^-1. Upon addition of oxygen, a significant increase in cathodic current occurred, likely due to electrocatalytic reduction of dioxygen to peroxide and/or water by CYP27B1. Characterization of the electrochemical properties of CYP27B1 is an important first step toward developing a bioelectrochemical method for measuring vitamin D in serum.

Original language	English
Pages (from-to)	1857-1860
Number of pages	4
Journal	Electrochemistry Communications
Volume	11
Issue number	10
DOIs	https://doi.org/10.1016/j.elecom.2009.08.002
State	Published - Oct 2009

Keywords

25-Hydroxyvitamin D
Cytochrome P450
Direct electrochemistry
Surfactant films
Thin-film voltammetry

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10.1016/j.elecom.2009.08.002

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title = "Direct electrochemistry of cytochrome P450 27B1 in surfactant films",

abstract = "We report the first direct electrochemistry of cytochrome P450 27B1 (CYP27B1) immobilized on an edge-plane pyrolytic graphite (EPG) electrode coated with a film of didodecyldimethylammonium bromide (DDAB). Cyclic voltammetry (CV) in a deoxygenated solution revealed excellent electrochemical reversibility with an average midpoint potential of -180 ± 5 mV vs. Ag/AgCl and an apparent surface coverage of (7.0 ± 2.5) × 1013 molecules per cm2. The rate of heterogeneous electron transfer between CYP27B1 and the EPG electrode was determined to be 3.5 ± 0.6 s-1. Upon addition of oxygen, a significant increase in cathodic current occurred, likely due to electrocatalytic reduction of dioxygen to peroxide and/or water by CYP27B1. Characterization of the electrochemical properties of CYP27B1 is an important first step toward developing a bioelectrochemical method for measuring vitamin D in serum.",

keywords = "25-Hydroxyvitamin D, Cytochrome P450, Direct electrochemistry, Surfactant films, Thin-film voltammetry",

author = "Rhieu, {Steve Y.} and Ludwig, {Daniel R.} and Siu, {Vince S.} and Palmore, {G. Tayhas R.}",

note = "Funding Information: We thank G.S. Reddy, MD for his help with HPLC analysis, Dr. T. Sakaki for generous provision of clones encoding mouse CYP27B1 and GroEL/ES. S.Y.R. and D.R.L. acknowledge financial support from the F.M. Becket Summer Fellowship of the Electrochemical Society and the NSF, respectively.",

year = "2009",

month = oct,

doi = "10.1016/j.elecom.2009.08.002",

language = "English",

volume = "11",

pages = "1857--1860",

journal = "Electrochemistry Communications",

issn = "1388-2481",

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T1 - Direct electrochemistry of cytochrome P450 27B1 in surfactant films

AU - Rhieu, Steve Y.

AU - Ludwig, Daniel R.

AU - Siu, Vince S.

AU - Palmore, G. Tayhas R.

N1 - Funding Information: We thank G.S. Reddy, MD for his help with HPLC analysis, Dr. T. Sakaki for generous provision of clones encoding mouse CYP27B1 and GroEL/ES. S.Y.R. and D.R.L. acknowledge financial support from the F.M. Becket Summer Fellowship of the Electrochemical Society and the NSF, respectively.

PY - 2009/10

Y1 - 2009/10

N2 - We report the first direct electrochemistry of cytochrome P450 27B1 (CYP27B1) immobilized on an edge-plane pyrolytic graphite (EPG) electrode coated with a film of didodecyldimethylammonium bromide (DDAB). Cyclic voltammetry (CV) in a deoxygenated solution revealed excellent electrochemical reversibility with an average midpoint potential of -180 ± 5 mV vs. Ag/AgCl and an apparent surface coverage of (7.0 ± 2.5) × 1013 molecules per cm2. The rate of heterogeneous electron transfer between CYP27B1 and the EPG electrode was determined to be 3.5 ± 0.6 s-1. Upon addition of oxygen, a significant increase in cathodic current occurred, likely due to electrocatalytic reduction of dioxygen to peroxide and/or water by CYP27B1. Characterization of the electrochemical properties of CYP27B1 is an important first step toward developing a bioelectrochemical method for measuring vitamin D in serum.

AB - We report the first direct electrochemistry of cytochrome P450 27B1 (CYP27B1) immobilized on an edge-plane pyrolytic graphite (EPG) electrode coated with a film of didodecyldimethylammonium bromide (DDAB). Cyclic voltammetry (CV) in a deoxygenated solution revealed excellent electrochemical reversibility with an average midpoint potential of -180 ± 5 mV vs. Ag/AgCl and an apparent surface coverage of (7.0 ± 2.5) × 1013 molecules per cm2. The rate of heterogeneous electron transfer between CYP27B1 and the EPG electrode was determined to be 3.5 ± 0.6 s-1. Upon addition of oxygen, a significant increase in cathodic current occurred, likely due to electrocatalytic reduction of dioxygen to peroxide and/or water by CYP27B1. Characterization of the electrochemical properties of CYP27B1 is an important first step toward developing a bioelectrochemical method for measuring vitamin D in serum.

KW - 25-Hydroxyvitamin D

KW - Cytochrome P450

KW - Direct electrochemistry

KW - Surfactant films

KW - Thin-film voltammetry

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U2 - 10.1016/j.elecom.2009.08.002

DO - 10.1016/j.elecom.2009.08.002

M3 - Article

AN - SCOPUS:70349754715

SN - 1388-2481

VL - 11

SP - 1857

EP - 1860

JO - Electrochemistry Communications

JF - Electrochemistry Communications

IS - 10

ER -

Direct electrochemistry of cytochrome P450 27B1 in surfactant films

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