Direct electrochemistry of cytochrome P450 27B1 in surfactant films

Steve Y. Rhieu, Daniel R. Ludwig, Vince S. Siu, G. Tayhas R. Palmore

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

We report the first direct electrochemistry of cytochrome P450 27B1 (CYP27B1) immobilized on an edge-plane pyrolytic graphite (EPG) electrode coated with a film of didodecyldimethylammonium bromide (DDAB). Cyclic voltammetry (CV) in a deoxygenated solution revealed excellent electrochemical reversibility with an average midpoint potential of -180 ± 5 mV vs. Ag/AgCl and an apparent surface coverage of (7.0 ± 2.5) × 1013 molecules per cm2. The rate of heterogeneous electron transfer between CYP27B1 and the EPG electrode was determined to be 3.5 ± 0.6 s-1. Upon addition of oxygen, a significant increase in cathodic current occurred, likely due to electrocatalytic reduction of dioxygen to peroxide and/or water by CYP27B1. Characterization of the electrochemical properties of CYP27B1 is an important first step toward developing a bioelectrochemical method for measuring vitamin D in serum.

Original languageEnglish
Pages (from-to)1857-1860
Number of pages4
JournalElectrochemistry Communications
Volume11
Issue number10
DOIs
StatePublished - Oct 2009

Keywords

  • 25-Hydroxyvitamin D
  • Cytochrome P450
  • Direct electrochemistry
  • Surfactant films
  • Thin-film voltammetry

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