Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells

Dechen Jiang; Anando Devadoss; M. Simona Palencsár; Danjun Fang; Nicole M. White; Thomas J. Kelley; Jonathan D. Smith; James D. Burgess

doi:10.1021/ja074373c

Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells

Dechen Jiang, Anando Devadoss, M. Simona Palencsár, Danjun Fang, Nicole M. White, Thomas J. Kelley, Jonathan D. Smith, James D. Burgess

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Cholesterol is a tightly regulated structural component of the cell plasma membrane. Dysfunctional intracellular cholesterol transport machinery causes disease states with altered plasma membrane cholesterol. This Communication reports microelectrode evaluation of plasma membrane cholesterol of single cells at physiological temperature. Electrochemical data indicate that transport of intracellular cholesterol to the plasma membrane is active in an atherosclerotic macrophage model.

Original language	English
Pages (from-to)	11352-11353
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	129
Issue number	37
DOIs	https://doi.org/10.1021/ja074373c
State	Published - Sep 19 2007

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title = "Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells",

abstract = "Cholesterol is a tightly regulated structural component of the cell plasma membrane. Dysfunctional intracellular cholesterol transport machinery causes disease states with altered plasma membrane cholesterol. This Communication reports microelectrode evaluation of plasma membrane cholesterol of single cells at physiological temperature. Electrochemical data indicate that transport of intracellular cholesterol to the plasma membrane is active in an atherosclerotic macrophage model.",

author = "Dechen Jiang and Anando Devadoss and Palencs{\'a}r, {M. Simona} and Danjun Fang and White, {Nicole M.} and Kelley, {Thomas J.} and Smith, {Jonathan D.} and Burgess, {James D.}",

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AU - Devadoss, Anando

AU - Palencsár, M. Simona

AU - Fang, Danjun

AU - White, Nicole M.

AU - Kelley, Thomas J.

AU - Smith, Jonathan D.

AU - Burgess, James D.

PY - 2007/9/19

Y1 - 2007/9/19

N2 - Cholesterol is a tightly regulated structural component of the cell plasma membrane. Dysfunctional intracellular cholesterol transport machinery causes disease states with altered plasma membrane cholesterol. This Communication reports microelectrode evaluation of plasma membrane cholesterol of single cells at physiological temperature. Electrochemical data indicate that transport of intracellular cholesterol to the plasma membrane is active in an atherosclerotic macrophage model.

AB - Cholesterol is a tightly regulated structural component of the cell plasma membrane. Dysfunctional intracellular cholesterol transport machinery causes disease states with altered plasma membrane cholesterol. This Communication reports microelectrode evaluation of plasma membrane cholesterol of single cells at physiological temperature. Electrochemical data indicate that transport of intracellular cholesterol to the plasma membrane is active in an atherosclerotic macrophage model.

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JO - Journal of the American Chemical Society

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Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells

Abstract

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