Diffuse optical tomography of cerebral blood flow, oxygenation, and metabolism in rat during focal ischemia

Joseph P. Culver, Turgut Durduran, Daisuke Furuya, Cecil Cheung, Joel H. Greenberg, A. G. Yodh

Research output: Contribution to journalArticlepeer-review

351 Scopus citations


Diffuse optical tomography (DOT) is an attractive approach for evaluating stroke physiology. It provides hemodynamic and metabolic imaging with unique potential for continuous noninvasive bedside imaging in humans. To date there have been few quantitative spatial-temporal studies of stroke pathophysiology based on diffuse optical signatures. The authors report DOT images of hemodynamic and metabolic contrasts using a rat middle cerebral artery occlusion (MCAO) stroke model. This study used a novel DOT device that concurrently obtains coregistered images of relative cerebral blood volume (rCBV), tissue-averaged hemoglobin oxygen saturation (Sto2), and relative cerebral blood flow (rCBF). The authors demonstrate how these hemodynamic measures can be synthesized to calculate an index of the oxygen extraction fraction (OEF) and the cerebral metabolic rate of oxygen consumption (CMRo2). Temporary (60-minute) MCAO was performed on five rats. Ischemic changes, averaged over the 60 minutes of occlusion, were as follows: rCBF = 0.42 ± 0.04, rCBV = 1.02 ± 0.04, ΔSto2 = -11 ± 2%, rOEF = 1.39 ± 0.06 and rCMRo2 = 0.59 ± 0.07. Although rOEF increased in response to decreased blood flow, rCMRo2 decreased. The sensitivity of this method of DOT analysis is discussed in terms of assumptions about baseline physiology, and the diffuse optical results are compared with positron emission tomography, magnetic resonance imaging, and histology observations in the literature.

Original languageEnglish
Pages (from-to)911-924
Number of pages14
JournalJournal of Cerebral Blood Flow and Metabolism
Issue number8
StatePublished - Aug 1 2003


  • Cerebral blood flow
  • Cerebral metabolic rate of oxygen consumption
  • Diffuse optical tomography
  • Ischemia
  • Laser Doppler flowmetry
  • Tissue oxygen saturation


Dive into the research topics of 'Diffuse optical tomography of cerebral blood flow, oxygenation, and metabolism in rat during focal ischemia'. Together they form a unique fingerprint.

Cite this