Differing roles of pyruvate dehydrogenase kinases during mouse oocyte maturation

Xiaojing Hou, Liang Zhang, Longsen Han, Juan Ge, Rujun Ma, Xuesen Zhang, Kelle Moley, Tim Schedl, Qiang Wang

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Pyruvate dehydrogenase kinases (PDKs) modulate energy homeostasis in multiple tissues and cell types, under various nutrient conditions, through phosphorylation of the α subunit (PDHE1α, also known as PDHA1) of the pyruvate dehydrogenase (PDH) complex. However, the roles of PDKs in meiotic maturation are currently unknown. Here, by undertaking knockdown and over expression analysis of PDK paralogs (PDK1-PDK4) in mouse oocytes, we established the site-specificity of PDKs towards the phosphorylation of three serine residues (Ser232,Ser293 and Ser300) on PDHE1α. We found that PDK3-mediated phosphorylation of Ser293-PDHE1α results in disruption of meiotic spindle morphology and chromosome alignment and decreased total ATP levels, probably through inhibition of PDH activity. Unexpectedly, we discovered that PDK1 and PDK2 promote meiotic maturation, as their knockdown disturbs the assembly of the meiotic apparatus, without significantly altering ATP content. Moreover, phosphorylation of Ser232-PDHE1α was demonstrated to mediate PDK1 and PDK2 action in meiotic maturation, possibly through a mechanism that is distinct from PDH inactivation. These findings reveal that there are divergent roles of PDKs during oocyte maturation and indicate a new mechanism controlling meiotic structure.

Original languageEnglish
Pages (from-to)2319-2329
Number of pages11
JournalJournal of cell science
Volume128
Issue number13
DOIs
StatePublished - Jan 1 2015

Keywords

  • Meiosis
  • Metabolism
  • Oocyte
  • Pyruvate dehydrogenase kinase
  • Spindle

Fingerprint Dive into the research topics of 'Differing roles of pyruvate dehydrogenase kinases during mouse oocyte maturation'. Together they form a unique fingerprint.

  • Cite this

    Hou, X., Zhang, L., Han, L., Ge, J., Ma, R., Zhang, X., Moley, K., Schedl, T., & Wang, Q. (2015). Differing roles of pyruvate dehydrogenase kinases during mouse oocyte maturation. Journal of cell science, 128(13), 2319-2329. https://doi.org/10.1242/jcs.167049