TY - JOUR
T1 - Differentiation of phagocytic monocytes into lymph node dendritic cells in vivo
AU - Randolph, Gwendalyn J.
AU - Inaba, Kayo
AU - Robbiani, Davide F.
AU - Steinman, Ralph M.
AU - Muller, William A.
N1 - Funding Information:
We are grateful to Hongli Li and Petra Hänel for expert technical assistance, to Frank Isdell and Dr. Regis Josien for advice and help with flow cytometry, and to Dr. Maggi Pack for advice on preparation of frozen sections and immunostaining. This work was supported by a National Institutes of Health National Research Service Award (HL09722) and American Heart Association (AHA) Scientist Development Grant (9930118N) to G. J. R., an award from De Bernardi Foundation of Bern Medical School (Switzerland) to D. F. R., and grants from the National Institutes of Health to R. M. S. (AI 13013) and W. A. M. (HL 46849). W. A. M. is an Established Investigator of the AHA.
PY - 1999/12
Y1 - 1999/12
N2 - We investigated the differentiation and trafficking of inflammatory monocytes that phagocytosed subcutaneously injected fluorescent microspheres. As expected, most of the monocytes became microsphere+ macrophages, which remained in subcutaneous tissue. However, about 25% of latex+ cells migrated to the T cell area of draining lymph nodes, where they expressed dendritic cell (DC)-restricted markers and high levels of costimulatory molecules. Microsphere-transporting cells were distinct from resident skin DCs, and this transport was reduced by more than 85% in monocyte-deficient osteopetrotic mice. Thus, a substantial minority of inflammatory monocytes carry phagocytosed particles to lymph nodes and differentiate into DCs.
AB - We investigated the differentiation and trafficking of inflammatory monocytes that phagocytosed subcutaneously injected fluorescent microspheres. As expected, most of the monocytes became microsphere+ macrophages, which remained in subcutaneous tissue. However, about 25% of latex+ cells migrated to the T cell area of draining lymph nodes, where they expressed dendritic cell (DC)-restricted markers and high levels of costimulatory molecules. Microsphere-transporting cells were distinct from resident skin DCs, and this transport was reduced by more than 85% in monocyte-deficient osteopetrotic mice. Thus, a substantial minority of inflammatory monocytes carry phagocytosed particles to lymph nodes and differentiate into DCs.
UR - http://www.scopus.com/inward/record.url?scp=0033403066&partnerID=8YFLogxK
U2 - 10.1016/S1074-7613(00)80149-1
DO - 10.1016/S1074-7613(00)80149-1
M3 - Article
C2 - 10626897
AN - SCOPUS:0033403066
SN - 1074-7613
VL - 11
SP - 753
EP - 761
JO - Immunity
JF - Immunity
IS - 6
ER -