Differentiation of phagocytic monocytes into lymph node dendritic cells in vivo

Gwendalyn J. Randolph, Kayo Inaba, Davide F. Robbiani, Ralph M. Steinman, William A. Muller

Research output: Contribution to journalArticle

723 Scopus citations

Abstract

We investigated the differentiation and trafficking of inflammatory monocytes that phagocytosed subcutaneously injected fluorescent microspheres. As expected, most of the monocytes became microsphere+ macrophages, which remained in subcutaneous tissue. However, about 25% of latex+ cells migrated to the T cell area of draining lymph nodes, where they expressed dendritic cell (DC)-restricted markers and high levels of costimulatory molecules. Microsphere-transporting cells were distinct from resident skin DCs, and this transport was reduced by more than 85% in monocyte-deficient osteopetrotic mice. Thus, a substantial minority of inflammatory monocytes carry phagocytosed particles to lymph nodes and differentiate into DCs.

Original languageEnglish
Pages (from-to)753-761
Number of pages9
JournalImmunity
Volume11
Issue number6
DOIs
StatePublished - Dec 1999
Externally publishedYes

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