Differentiation of Aedes triseriatus (Say) from Aedes hendersoni cockerell (Diptera: Culicidae) by restriction fragment length polymorphisms of amplified ribosomal DNA

H. E. Reno, M. H. Vodkin, R. J. Novak

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Aedes triseriatus is the primary vector of LaCrosse (LAC) virus, which can cause encephalitis, especially in young children. Aedes hendersoni, a sibling species of Ae. triseriatus, has a salivary gland barrier to LAC virus and, therefore, is not considered a vector of this virus. Adults of Ae. triseriatus are morphologically indistinguishable from those of Ae. hendersoni, and the two species are sympatric in the eastern United States. A definitive method of identifying field specimens is an important part of any disease surveillance program, particularly in the case of LAC virus. This study identifies restriction enzymes that produce species-specific restriction fragment length polymorphisms (RFLPs) from amplified ribosomal (r) DNA. In addition, sequences of the internal transcribed spacers 1 and 2 and the 5.8S regions of the rDNA were used to confirm the RFLP patterns. This study is the first to compare nucleotide sequences from Ae. triseriatus and Ae. hendersoni.

Original languageEnglish
Pages (from-to)193-199
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume62
Issue number2
DOIs
StatePublished - Feb 2000
Externally publishedYes

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