@article{60e107aab0a24aa1bd60db2c580e5348,
title = "Differentiation and maturation of oligodendrocytes in human three-dimensional neural cultures",
abstract = "Investigating human oligodendrogenesis and the interaction of oligodendrocytes with neurons and astrocytes would accelerate our understanding of the mechanisms underlying white matter disorders. However, this is challenging because of the limited accessibility of functional human brain tissue. Here, we developed a new differentiation method of human induced pluripotent stem cells to generate three-dimensional brain organoids that contain oligodendrocytes as well as neurons and astrocytes, called human oligodendrocyte spheroids. We found that oligodendrocyte lineage cells derived in human oligodendrocyte spheroids transitioned through developmental stages similar to primary human oligodendrocytes and that the migration of oligodendrocyte lineage cells and their susceptibility to lysolecithin exposure could be captured by live imaging. Moreover, their morphology changed as they matured over time in vitro and started myelinating neurons. We anticipate that this method can be used to study oligodendrocyte development, myelination, and interactions with other major cell types in the CNS.",
author = "Marton, {Rebecca M.} and Yuki Miura and Sloan, {Steven A.} and Qingyun Li and Omer Revah and Levy, {Rebecca J.} and Huguenard, {John R.} and Pașca, {Sergiu P.}",
note = "Funding Information: This paper is dedicated to the memory of our wonderful colleague and mentor Ben A. Barres. We thank B.A. Barres, B. Zuchero, and members of the Pasca laboratory for scientific input, J. Perrino (Stanford Cell Sciences Imaging Facility) for support with electron microscopy, as well as F. Sim (University of Buffalo) for providing the Sox10-MCS5::eGFP plasmid. This work was supported by the US National Institutes of Health BRAINS Award (MH107800), the MQ Fellow Award, the NYSCF Robertson Stem Cell Investigator Award, the Stanford Wu Tsai Neurosciences Institute{\textquoteright}s Brain Rejuvenation Project and the Human Brain Organogenesis Project, the Kwan Research Fund and the California Institute of Regenerative Medicine, the Child Health Research Institute Pilot Award, and the NARSAD Independent Investigator Award from the Brain and Behavior Research Foundation (to S.P.P.); the National Science Foundation Graduate Research Fellowship and the Bio-X Stanford Interdisciplinary Graduate Fellowship (to R.M.M.); Stanford Medicine{\textquoteright}s Dean{\textquoteright}s Fellowship (to Y.M.); and NIMH T32GM007365, F30MH106261, and Bio-X Predoctoral Fellowship (to or supporting S.A.S.). Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.",
year = "2019",
month = mar,
day = "1",
doi = "10.1038/s41593-018-0316-9",
language = "English",
volume = "22",
pages = "484--491",
journal = "Nature neuroscience",
issn = "1097-6256",
number = "3",
}