Differential usage of transcriptional repressor Zeb2 enhancers distinguishes adult and embryonic hematopoiesis

Xiao Huang, Stephen T. Ferris, Sunkyung Kim, Mayank N.K. Choudhary, Julia A. Belk, Changxu Fan, Yanyan Qi, Raki Sudan, Yu Xia, Pritesh Desai, Jing Chen, Nghi Ly, Quanming Shi, Prachi Bagadia, Tiantian Liu, Martin Guilliams, Takeshi Egawa, Marco Colonna, Michael S. Diamond, Theresa L. MurphyAnsuman T. Satpathy, Ting Wang, Kenneth M. Murphy

Research output: Contribution to journalArticlepeer-review

Abstract

The transcriptional repressor ZEB2 regulates development of many cell fates among somatic, neural, and hematopoietic lineages, but the basis for its requirement in these diverse lineages is unclear. Here, we identified a 400-basepair (bp) region located 165 kilobases (kb) upstream of the Zeb2 transcriptional start site (TSS) that binds the E proteins at several E-box motifs and was active in hematopoietic lineages. Germline deletion of this 400-bp region (Zeb2Δ−165mice) specifically prevented Zeb2 expression in hematopoietic stem cell (HSC)-derived lineages. Zeb2Δ−165 mice lacked development of plasmacytoid dendritic cells (pDCs), monocytes, and B cells. All macrophages in Zeb2Δ−165 mice were exclusively of embryonic origin. Using single-cell chromatin profiling, we identified a second Zeb2 enhancer located at +164-kb that was selectively active in embryonically derived lineages, but not HSC-derived ones. Thus, Zeb2 expression in adult, but not embryonic, hematopoiesis is selectively controlled by the –165-kb Zeb2 enhancer.

Original languageEnglish
Pages (from-to)1417-1432.e7
JournalImmunity
Volume54
Issue number7
DOIs
StatePublished - Jul 13 2021

Keywords

  • Zeb2
  • chromatin structure
  • enhancer
  • hematopoiesis
  • transcriptional regulation
  • zinc finger E-box binding homeobox 2

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