TY - JOUR
T1 - Differential interaction of the two cholesterol-dependent, membrane-damaging toxins, streptolysin O and Vibrio cholerae cytolysin, with enantiomeric cholesterol
AU - Zitzer, Alexander
AU - Westover, Emily J.
AU - Covey, Douglas F.
AU - Palmer, Michael
N1 - Funding Information:
We thank Robert Bittman and Evan Mintzer for useful discussions. This work was supported by NSERC grant No. 250265-2003 GSC-032 (to M.P.), NIH GM47969 (to D.F.C.), the Lucille P. Markey Predoctoral Fellowship and Cardiovascular Research Training Grant NIH 55935 (to E.J.W.).
PY - 2003/10/23
Y1 - 2003/10/23
N2 - Membrane cholesterol is essential to the activity of at least two structurally unrelated families of bacterial pore-forming toxins, represented by streptolysin O (SLO) and Vibrio cholerae cytolysin (VCC), respectively. Here, we report that SLO and VCC differ sharply in their interaction with liposome membranes containing enantiomeric cholesterol (ent-cholesterol). VCC had very low activity with ent-cholesterol, which is in line with a stereospecific mode of interaction of this toxin with cholesterol. In contrast, SLO was only slightly less active with ent-cholesterol than with cholesterol, suggesting a rather limited degree of structural specificity in the toxin-cholesterol interaction.
AB - Membrane cholesterol is essential to the activity of at least two structurally unrelated families of bacterial pore-forming toxins, represented by streptolysin O (SLO) and Vibrio cholerae cytolysin (VCC), respectively. Here, we report that SLO and VCC differ sharply in their interaction with liposome membranes containing enantiomeric cholesterol (ent-cholesterol). VCC had very low activity with ent-cholesterol, which is in line with a stereospecific mode of interaction of this toxin with cholesterol. In contrast, SLO was only slightly less active with ent-cholesterol than with cholesterol, suggesting a rather limited degree of structural specificity in the toxin-cholesterol interaction.
KW - Cholesterol-binding cytolysins
KW - Enantiomeric cholesterol
KW - Protein-cholesterol interaction
KW - Streptolysin O
KW - Vibrio cholerae cytolysin
UR - http://www.scopus.com/inward/record.url?scp=0142231450&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(03)01023-8
DO - 10.1016/S0014-5793(03)01023-8
M3 - Article
C2 - 14572629
AN - SCOPUS:0142231450
SN - 0014-5793
VL - 553
SP - 229
EP - 231
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -