Differential expression of Low density lipoprotein Receptor–related Protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions

Mónica B. Gilardoni, María M. Remedi, Mabel Oviedo, Tristán Dellavedova, Juan P. Sarría, Laura Racca, Mariana Dominguez, Claudia G. Pellizas, Ana C. Donadio

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Background Metalloproteinases (MMPs) are relevant modulators of inflammation, tumor microenvironment, cancer invasion and metastasis. They can be regulated by the Low density lipoprotein Receptor–related Protein 1 (LRP-1), a receptor reported to mediate the clearance of lipoproteins, extracellular matrix (ECM) macromolecules and proteinases. The aim of this study was to evaluate the expression of LRP-1, MMP-2 and MMP-9 across various grades of prostatic diseases as benign prostatic hyperplasia (BPH), BPH plus prostatitis (BPH + P), high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer (PCa). Methods LRP-1 was analyzed using immunohistochemistry and MMPs proteolytic activity by zymography in prostate tissues with different prostatic diseases. Results LRP-1 was detected in epithelial cells in BPH (16/18), BPH + P (21/21) and HGPIN (6/6), with a staining intensity of 1+, 1+/2+ and 3+, respectively. In PCa, LRP-1 was absent in 19/27 samples while a low expression was observed in 8/27 biopsies. MMP-9 activity was higher and statistically significant in PCa than in BPH (p ≤ 0.01). Conclusion Considering that LRP-1, by mediating the clearance of MMPs, is involved in the regulation of ECM remodeling and cell migration, we conclude that a decreased expression of LRP-1 could be involved with the increasing activity of MMPs shown in cancers.

Original languageEnglish
Pages (from-to)66-71
Number of pages6
JournalPathology Research and Practice
Volume213
Issue number1
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Keywords

  • Cancer
  • Immunohistochemistry
  • LRP-1
  • MMP-9
  • Prostate
  • Zymography

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