Differential expression of biological effects in maturationally distinct subpopulations of growth plate chondrocytes

Regis J. O'keefe; Randy N. Rosier; J. Edward Puzas

doi:10.3109/03008209009152422

Differential expression of biological effects in maturationally distinct subpopulations of growth plate chondrocytes

Regis J. O'keefe, Randy N. Rosier, J. Edward Puzas

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10 Scopus citations

Abstract

Countercurrent centrifugal elutriation is an accurate and reproducible technique which can separate cells on the basis of size. This technique was utilized for the separation of isolated chick growth plate chondrocytes. Mean cellular volume, alkaline phosphatase activity, and Type X collagen synthesis progressively increased in each of seven successive separated fractions. The chondrocytes from the different fractions expressed a differential response to the growth regulator, transforming growth factor beta (TGF β TGF β receptor binding to these cell fractions suggests that the different biological effects may be due to differences in the number of receptors expressed per cell as well as to differences in the proportion of high and low affinity TGF β receptors present.

Original language	English
Pages (from-to)	53-66
Number of pages	14
Journal	Connective Tissue Research
Volume	24
Issue number	1
DOIs	https://doi.org/10.3109/03008209009152422
State	Published - 1990

Keywords

Cell separation
Chondrocyte
Elutriation
Receptor binding
TGF β

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10.3109/03008209009152422

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title = "Differential expression of biological effects in maturationally distinct subpopulations of growth plate chondrocytes",

abstract = "Countercurrent centrifugal elutriation is an accurate and reproducible technique which can separate cells on the basis of size. This technique was utilized for the separation of isolated chick growth plate chondrocytes. Mean cellular volume, alkaline phosphatase activity, and Type X collagen synthesis progressively increased in each of seven successive separated fractions. The chondrocytes from the different fractions expressed a differential response to the growth regulator, transforming growth factor beta (TGF β TGF β receptor binding to these cell fractions suggests that the different biological effects may be due to differences in the number of receptors expressed per cell as well as to differences in the proportion of high and low affinity TGF β receptors present.",

keywords = "Cell separation, Chondrocyte, Elutriation, Receptor binding, TGF β",

author = "O'keefe, {Regis J.} and Rosier, {Randy N.} and Puzas, {J. Edward}",

note = "Funding Information: The authors acknowledge the technical assistance of Laura Hansen and Brenda King. This work was supported in pprt by NIH T32AM07092-12, NIH AR 38945, NIH AR 38618 an NIH AR 28420.",

year = "1990",

doi = "10.3109/03008209009152422",

language = "English",

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pages = "53--66",

journal = "Connective Tissue Research",

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T1 - Differential expression of biological effects in maturationally distinct subpopulations of growth plate chondrocytes

AU - O'keefe, Regis J.

AU - Rosier, Randy N.

AU - Puzas, J. Edward

N1 - Funding Information: The authors acknowledge the technical assistance of Laura Hansen and Brenda King. This work was supported in pprt by NIH T32AM07092-12, NIH AR 38945, NIH AR 38618 an NIH AR 28420.

PY - 1990

Y1 - 1990

N2 - Countercurrent centrifugal elutriation is an accurate and reproducible technique which can separate cells on the basis of size. This technique was utilized for the separation of isolated chick growth plate chondrocytes. Mean cellular volume, alkaline phosphatase activity, and Type X collagen synthesis progressively increased in each of seven successive separated fractions. The chondrocytes from the different fractions expressed a differential response to the growth regulator, transforming growth factor beta (TGF β TGF β receptor binding to these cell fractions suggests that the different biological effects may be due to differences in the number of receptors expressed per cell as well as to differences in the proportion of high and low affinity TGF β receptors present.

AB - Countercurrent centrifugal elutriation is an accurate and reproducible technique which can separate cells on the basis of size. This technique was utilized for the separation of isolated chick growth plate chondrocytes. Mean cellular volume, alkaline phosphatase activity, and Type X collagen synthesis progressively increased in each of seven successive separated fractions. The chondrocytes from the different fractions expressed a differential response to the growth regulator, transforming growth factor beta (TGF β TGF β receptor binding to these cell fractions suggests that the different biological effects may be due to differences in the number of receptors expressed per cell as well as to differences in the proportion of high and low affinity TGF β receptors present.

KW - Cell separation

KW - Chondrocyte

KW - Elutriation

KW - Receptor binding

KW - TGF β

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DO - 10.3109/03008209009152422

M3 - Article

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JO - Connective Tissue Research

JF - Connective Tissue Research

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ER -

Differential expression of biological effects in maturationally distinct subpopulations of growth plate chondrocytes

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