Mononuclear phagocytes present in various tissues are capable of extensive proliferation and form discrete colonies in vitro under appropriate culture conditions. In this study, the effect of hemolysates on the clonal growth of various classes of mononuclear phagocyte colony-forming cells (CFC) was investigated by using a double-layer agar culture technique. We added graded doses of mouse, hamster, rabbit or rat hemolysate to the feeder layer. The results showed that hemolysates can greatly increase the cloning efficiency of CFC in the blood (BL) (10 to 65 fold) but only slightly the CFC in alveolar spaces. The increase in the cloning efficiency of CFC in peritoneal exudate (PE) was about 2 to 3-fold. Moreover, hemolysate significantly reduced the length of the initial lag period for colony formation by PE-CFC and BL-CFC. The active substance present in the hemolysate was nondialysable, heat labile and resistant to either treatment.
|Number of pages||8|
|Journal||RES Journal of the Reticuloendothelial Society|
|State||Published - 1981|