TY - JOUR
T1 - Differential effects of intra- and extraluminal endothelin on cerebral arterioles
AU - Ogura, K.
AU - Takayasu, M.
AU - Dacey, R. G.
PY - 1991
Y1 - 1991
N2 - The differential effects of intraluminal and extraluminal application of endothelin, the endothelium-derived constricting factor, were studied in isolated intracerebral arterioles that were cannulated and perfused in vitro. Extraluminal and intraluminal application of 10-8 M endothelin constricted the arterioles to 38.2 ± 6.7 and 75.1 ± 5.7% of the control diameter, respectively. Constrictions induced by either the intraluminal or the extraluminal application of endothelin were inhibited in a Ca2+-free solution. Nimodipine attenuated, but did not completely abolish, the constriction induced by the extraluminal application of endothelin, but it did abolish the constriction induced by the intraluminal application. The duration of vasoconstriction also differed between intra- and extraluminal application. Vessel diameter quickly returned to the control value as soon as intraluminal perfusion of endothelin was terminated. In contrast, the constriction induced by extraluminal application of endothelin lasted much longer and was difficult to reverse. The differential effect of intraluminal and extraluminal endothelin application appears to reflect the important barrier-function of the cerebrovascular endothelium of intracerebral arterioles.
AB - The differential effects of intraluminal and extraluminal application of endothelin, the endothelium-derived constricting factor, were studied in isolated intracerebral arterioles that were cannulated and perfused in vitro. Extraluminal and intraluminal application of 10-8 M endothelin constricted the arterioles to 38.2 ± 6.7 and 75.1 ± 5.7% of the control diameter, respectively. Constrictions induced by either the intraluminal or the extraluminal application of endothelin were inhibited in a Ca2+-free solution. Nimodipine attenuated, but did not completely abolish, the constriction induced by the extraluminal application of endothelin, but it did abolish the constriction induced by the intraluminal application. The duration of vasoconstriction also differed between intra- and extraluminal application. Vessel diameter quickly returned to the control value as soon as intraluminal perfusion of endothelin was terminated. In contrast, the constriction induced by extraluminal application of endothelin lasted much longer and was difficult to reverse. The differential effect of intraluminal and extraluminal endothelin application appears to reflect the important barrier-function of the cerebrovascular endothelium of intracerebral arterioles.
KW - Blood-brain barrier
KW - Calcium
KW - Cerebral Endothelium-derived constricting factor
KW - Nimodipine
KW - Vascular smooth muscle
UR - http://www.scopus.com/inward/record.url?scp=0025948514&partnerID=8YFLogxK
U2 - 10.1152/ajpheart.1991.261.2.h531
DO - 10.1152/ajpheart.1991.261.2.h531
M3 - Article
C2 - 1877678
AN - SCOPUS:0025948514
SN - 0002-9513
VL - 261
SP - H531-H537
JO - American Journal of Physiology - Heart and Circulatory Physiology
JF - American Journal of Physiology - Heart and Circulatory Physiology
IS - 2 30-2
ER -