TY - JOUR
T1 - Differential cytotoxicity of metal oxide nanoparticles
AU - Chen, Jian
AU - Zhu, Jinmin
AU - Cho, Hyun Hee
AU - Cui, Kemi
AU - Li, Fuhai
AU - Zhou, Xiaobo
AU - Rogers, Jack T.
AU - Wong, Stephen T.C.
AU - Huang, Xudong
N1 - Funding Information:
This work was supported by a NIH Career Development grant (X. Huang, 5K01MH002001) and funds from Radiology Department of Brigham and Women’s Hospital, the Center for Bioinformatics Program Grant (S.T.C. Wong) of Harvard Center for Neurodegeneration & Repair. J. Zhu is a recipient of a K12 training grant (D. N. Kennedy, 5K12MH069281).
PY - 2008
Y1 - 2008
N2 - Concerns about the potential health hazards of nanomaterials are growing. To determine the potential toxicity of metal oxide nanoparticles, human SH-SY5Y neuroblastoma and H4 neuroglioma cells were exposed to Fe2O3, CuO and ZnO nanoparticles and their metal ion counterparts (Fe3+, Cu2+ and Zn3+) at a concentration range of 0.01-100 μM for 48 h, under the cell culture conditions: 95% O2, 5% CO2, 85% humidity, 37°C. Their ensemble cell viability was determined by MTS cell proliferation assay. A live/dead cell assay was also performed, and cellular images were acquired by a high-content fluorescence microscope and quantified by a novel computerised image analysis protocol. Our data indicated that exposure of these nanoparticles induced differential toxic effects in both SH-SY5Y and H4 cells, and the cells had dose-dependent toxic responses to the CuO nanoparticle insult. In conclusion, the toxic responses of the nanoparticles are complex, and they warrant further in vivo studies. However, it remains to be determined if these nanopartilces have synergistically enhancing or cancelling toxic effects upon both SH-SY5Y and H4 cells.
AB - Concerns about the potential health hazards of nanomaterials are growing. To determine the potential toxicity of metal oxide nanoparticles, human SH-SY5Y neuroblastoma and H4 neuroglioma cells were exposed to Fe2O3, CuO and ZnO nanoparticles and their metal ion counterparts (Fe3+, Cu2+ and Zn3+) at a concentration range of 0.01-100 μM for 48 h, under the cell culture conditions: 95% O2, 5% CO2, 85% humidity, 37°C. Their ensemble cell viability was determined by MTS cell proliferation assay. A live/dead cell assay was also performed, and cellular images were acquired by a high-content fluorescence microscope and quantified by a novel computerised image analysis protocol. Our data indicated that exposure of these nanoparticles induced differential toxic effects in both SH-SY5Y and H4 cells, and the cells had dose-dependent toxic responses to the CuO nanoparticle insult. In conclusion, the toxic responses of the nanoparticles are complex, and they warrant further in vivo studies. However, it remains to be determined if these nanopartilces have synergistically enhancing or cancelling toxic effects upon both SH-SY5Y and H4 cells.
KW - Cytotoxicity
KW - H4 neuroglioma cell
KW - High-content cell imaging
KW - Metal oxide nanoparticles
KW - SH-SY5Y neuroblastoma cell
UR - http://www.scopus.com/inward/record.url?scp=65449128102&partnerID=8YFLogxK
U2 - 10.1080/17458080802235765
DO - 10.1080/17458080802235765
M3 - Article
AN - SCOPUS:65449128102
SN - 1745-8080
VL - 3
SP - 321
EP - 328
JO - Journal of Experimental Nanoscience
JF - Journal of Experimental Nanoscience
IS - 4
ER -