TY - JOUR
T1 - Different Mixed Astrocyte Populations Derived from Embryonic Stem Cells Have Variable Neuronal Growth Support Capacities
AU - Thompson, Russell E.
AU - Lake, Allison
AU - Kenny, Peter
AU - Saunders, Michael N.
AU - Sakers, Kristina
AU - Iyer, Nisha R.
AU - Dougherty, Joseph D.
AU - Sakiyama-Elbert, Shelly E.
N1 - Funding Information:
The technical expertise of Petra Erdmann-Gilmore is gratefully acknowledged. This work was supported by NINDS R01 NS090617-02, MH099798-01, the Washington University Medical Scientist Training Program (NIH T32 GM07200) and graduate program (T32 GM008151), and the Children’s Discovery Institute CDI (MD-II-2013-269). The LC-MS analysis and protein identifications were performed at the Washington University Proteomics Shared Resource (WU-PSR). The WU-PSR is supported by the WU Institute of Clinical and Translational Sciences (NCATS UL1 TR000448), the WU Mass Spectrometry Research Resource (NIGMS P41 GM103422), and the Siteman Comprehensive Cancer Center (NCI P30 CA091842). Sequencing of Illu-mina libraries was performed by the Washington University Genome Technology Access Center (supported by NIH grants P30 CA91842 and UL1TR000448).
Publisher Copyright:
© Copyright 2017, Mary Ann Liebert, Inc. 2017.
PY - 2017/11/15
Y1 - 2017/11/15
N2 - Central nervous system injury often leads to functional impairment due, in part, to the formation of an inhibitory glial scar following injury that contributes to poor regeneration. Astrocytes are the major cellular components of the glial scar, which has led to the belief that they are primarily inhibitory following injury. Recent work has challenged this by demonstrating that some astrocytes are required for spinal cord regeneration and astrocytic roles in recovery depend on their phenotype. In this work, two mixed populations containing primarily either fibrous or protoplasmic astrocytes were derived from mouse embryonic stem cells (mESCs). Motoneuron and V2a interneuron growth on live cultures, freeze-lysed cultures, or decellularized extracellular matrix (ECM) from astrocytes were assessed. Both neuronal populations were found to extend significantly longer neurites on protoplasmic-derived substrates than fibrous-derived substrates. Interestingly, neurons extended longer neurites on protoplasmic-derived ECM than fibrous-derived ECM. ECM proteins were compared with in vivo astrocyte expression profiles, and it was found that the ESC-derived ECMs were enriched for astrocyte-specific proteins. Further characterization revealed that protoplasmic ECM had significantly higher levels of axon growth promoting proteins, while fibrous ECM had significantly higher levels of proteins that inhibit axon growth. Supporting this observation, knockdown of spondin-1 improved neurite growth on fibrous ECM, while laminin α5 and γ1 knockdown decreased neurite growth on protoplasmic ECM. These methods allow for scalable production of specific astrocyte subtype-containing populations with different neuronal growth support capacities, and can be used for further studies of the functional importance of astrocyte heterogeneity.
AB - Central nervous system injury often leads to functional impairment due, in part, to the formation of an inhibitory glial scar following injury that contributes to poor regeneration. Astrocytes are the major cellular components of the glial scar, which has led to the belief that they are primarily inhibitory following injury. Recent work has challenged this by demonstrating that some astrocytes are required for spinal cord regeneration and astrocytic roles in recovery depend on their phenotype. In this work, two mixed populations containing primarily either fibrous or protoplasmic astrocytes were derived from mouse embryonic stem cells (mESCs). Motoneuron and V2a interneuron growth on live cultures, freeze-lysed cultures, or decellularized extracellular matrix (ECM) from astrocytes were assessed. Both neuronal populations were found to extend significantly longer neurites on protoplasmic-derived substrates than fibrous-derived substrates. Interestingly, neurons extended longer neurites on protoplasmic-derived ECM than fibrous-derived ECM. ECM proteins were compared with in vivo astrocyte expression profiles, and it was found that the ESC-derived ECMs were enriched for astrocyte-specific proteins. Further characterization revealed that protoplasmic ECM had significantly higher levels of axon growth promoting proteins, while fibrous ECM had significantly higher levels of proteins that inhibit axon growth. Supporting this observation, knockdown of spondin-1 improved neurite growth on fibrous ECM, while laminin α5 and γ1 knockdown decreased neurite growth on protoplasmic ECM. These methods allow for scalable production of specific astrocyte subtype-containing populations with different neuronal growth support capacities, and can be used for further studies of the functional importance of astrocyte heterogeneity.
KW - interneuron
KW - motor neuron
KW - spinal cord injury
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85033595823&partnerID=8YFLogxK
U2 - 10.1089/scd.2017.0121
DO - 10.1089/scd.2017.0121
M3 - Article
C2 - 28851266
AN - SCOPUS:85033595823
SN - 1547-3287
VL - 26
SP - 1597
EP - 1611
JO - Stem Cells and Development
JF - Stem Cells and Development
IS - 22
ER -