TY - JOUR
T1 - Differences in the response of several cell types to inhibition of surface receptor mobility by local concanavalina binding
AU - Henis, Yoav I.
AU - Elson, Elliot L.
N1 - Funding Information:
We thank Dr David Segal for the P388D, cells. This research was supported by NIH Grant GM 21661, and by a Chaim Weizmann post-doctoral fellowship to Y. I. H. The Washington University Center for Basic Cancer Research (funded by DHEW Grant 5P30 CA 16217) provided tissue culture media.
PY - 1981/11
Y1 - 1981/11
N2 - Concanavalin A (conA) modulates the lateral mobility of cell surface receptors differently on different cell types. This was demonstrated by using fluorescence photobleaching recovery (FPR) to measure the inhibition of the lateral mobility of conA receptors by localized binding of conA on lymphocytes, fibroblasts, and macrophages. On mouse spleen lymphocytes, binding of conA platelets above a threshold coverage (about 12% of the upper cell-surface area) reduced the diffusion coefficient of mobile TMR-SconA-receptor complexes from 3.0×10-10 cm2/sec to 0.6× 10-10 cm2/sec (a 5-fold decrease), and the fraction of mobile receptors was concomitantly reduced from 0.4 to 0.11. Below the threshold occupancy, no effect on either parameter was detected. On 3T3 cells, a qualitatively similar threshold phenomenon was observed: coverage of over 9% of the upper cell surface by conA platelets induced a 3-fold reduction in the diffusion coefficient of TMR-SconA-receptor complexes from 5×10-10 cm2/sec to 1.7× 10-10 cm2/sec. However, no effect on the mobile fraction (about 0.4) was observed. In contrast, neither the diffusion coefficient nor the mobile fraction of TMR-SconA-receptor complexes on mouse peritoneal macrophages (both resident and thioglycolate-stimulated) or on the mouse macrophage cell line P388D1 were affected by the binding of conA platelets in amounts covering over 50% of the upper cell surface (approx. 4.6× 10-10 cm2/sec and 0.5 for the diffusion coefficient and mobile fraction, respectively). These differences are correlated to the different cytoskeletal functions of the various cell types studied, and are discussed regarding the mechanism of the conA-induced modulation.
AB - Concanavalin A (conA) modulates the lateral mobility of cell surface receptors differently on different cell types. This was demonstrated by using fluorescence photobleaching recovery (FPR) to measure the inhibition of the lateral mobility of conA receptors by localized binding of conA on lymphocytes, fibroblasts, and macrophages. On mouse spleen lymphocytes, binding of conA platelets above a threshold coverage (about 12% of the upper cell-surface area) reduced the diffusion coefficient of mobile TMR-SconA-receptor complexes from 3.0×10-10 cm2/sec to 0.6× 10-10 cm2/sec (a 5-fold decrease), and the fraction of mobile receptors was concomitantly reduced from 0.4 to 0.11. Below the threshold occupancy, no effect on either parameter was detected. On 3T3 cells, a qualitatively similar threshold phenomenon was observed: coverage of over 9% of the upper cell surface by conA platelets induced a 3-fold reduction in the diffusion coefficient of TMR-SconA-receptor complexes from 5×10-10 cm2/sec to 1.7× 10-10 cm2/sec. However, no effect on the mobile fraction (about 0.4) was observed. In contrast, neither the diffusion coefficient nor the mobile fraction of TMR-SconA-receptor complexes on mouse peritoneal macrophages (both resident and thioglycolate-stimulated) or on the mouse macrophage cell line P388D1 were affected by the binding of conA platelets in amounts covering over 50% of the upper cell surface (approx. 4.6× 10-10 cm2/sec and 0.5 for the diffusion coefficient and mobile fraction, respectively). These differences are correlated to the different cytoskeletal functions of the various cell types studied, and are discussed regarding the mechanism of the conA-induced modulation.
UR - http://www.scopus.com/inward/record.url?scp=0019803399&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(81)90050-1
DO - 10.1016/0014-4827(81)90050-1
M3 - Article
C2 - 7297611
AN - SCOPUS:0019803399
SN - 0014-4827
VL - 136
SP - 189
EP - 201
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -