Differences in the regulation of K-Ras and H-Ras isoforms by monoubiquitination

Rachael Baker, Emily M. Wilkerson, Kazutaka Sumita, Daniel G. Isom, Atsuo T. Sasaki, Henrik G. Dohlman, Sharon L. Campbell

Research output: Contribution to journalArticlepeer-review

60 Scopus citations


Background: Ras proteins are critical regulators of cellular growth and are differentially modified by ubiquitination. Results: Chemical ubiquitination and immunoprecipitation assays demonstrate that monoubiquitination causes sustained H-Ras activation in the absence of oncogenic mutations. Conclusion: The mechanism by which H-Ras is activated by monoubiquitination is both isoform-specific and site-specific. Significance: Monoubiquitination adds a new level of regulation and complexity to isoform-specific Ras signaling.

Original languageEnglish
Pages (from-to)36856-36862
Number of pages7
JournalJournal of Biological Chemistry
Issue number52
StatePublished - Dec 27 2013


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