Difference in biophysical properties of cancer-initiating cells in melanoma mutated zebrafish

N. Makarova; Vivek Kalaparthi; Andrew Wang; Chris Williams; M. E. Dokukin; Charles K. Kaufman; Leonard Zon; I. Sokolov

doi:10.1016/j.jmbbm.2020.103746

Difference in biophysical properties of cancer-initiating cells in melanoma mutated zebrafish

N. Makarova, Vivek Kalaparthi, Andrew Wang, Chris Williams, M. E. Dokukin, Charles K. Kaufman, Leonard Zon, I. Sokolov

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Despite sharing oncogenetic mutations, only a small number of cells within a given tissue will undergo malignant transformation. Biochemical and physical factors responsible for this cancer-initiation process are not well understood. Here we study biophysical differences of pre-melanoma and melanoma cells in a BRAF^V600E/P53 zebrafish model. The AFM indentation technique was used to study the cancer-initiating cells while the surrounding melanocytes were the control. We observed a statistically significant decrease in the modulus of elasticity (the effective Young's modulus) of cancer-initiating cells compared to the surrounding melanocytes. No significant differences in the pericellular coat surrounding cells were observed. These results contribute to a better understanding of the factors responsible for the initiation of cancer.

Original language	English
Article number	103746
Journal	Journal of the Mechanical Behavior of Biomedical Materials
Volume	107
DOIs	https://doi.org/10.1016/j.jmbbm.2020.103746
State	Published - Jul 2020

Keywords

Atomic force microscopy
Cell mechanics
Initiation of cancer
Pericellular coat
Zebrafish

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10.1016/j.jmbbm.2020.103746

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title = "Difference in biophysical properties of cancer-initiating cells in melanoma mutated zebrafish",

abstract = "Despite sharing oncogenetic mutations, only a small number of cells within a given tissue will undergo malignant transformation. Biochemical and physical factors responsible for this cancer-initiation process are not well understood. Here we study biophysical differences of pre-melanoma and melanoma cells in a BRAFV600E/P53 zebrafish model. The AFM indentation technique was used to study the cancer-initiating cells while the surrounding melanocytes were the control. We observed a statistically significant decrease in the modulus of elasticity (the effective Young's modulus) of cancer-initiating cells compared to the surrounding melanocytes. No significant differences in the pericellular coat surrounding cells were observed. These results contribute to a better understanding of the factors responsible for the initiation of cancer.",

keywords = "Atomic force microscopy, Cell mechanics, Initiation of cancer, Pericellular coat, Zebrafish",

author = "N. Makarova and Vivek Kalaparthi and Andrew Wang and Chris Williams and Dokukin, {M. E.} and Kaufman, {Charles K.} and Leonard Zon and I. Sokolov",

note = "Funding Information: Support of this work from the National Science Foundation is acknowledged by I.S. (CMMI 1435655 , CMMI 1937373 ) and from the National Institutes of Health by L.Z. ( R01 CA103846 , P01 CA163222 ). Publisher Copyright: {\textcopyright} 2020",

year = "2020",

month = jul,

doi = "10.1016/j.jmbbm.2020.103746",

language = "English",

volume = "107",

journal = "Journal of the Mechanical Behavior of Biomedical Materials",

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T1 - Difference in biophysical properties of cancer-initiating cells in melanoma mutated zebrafish

AU - Makarova, N.

AU - Kalaparthi, Vivek

AU - Wang, Andrew

AU - Williams, Chris

AU - Dokukin, M. E.

AU - Kaufman, Charles K.

AU - Zon, Leonard

AU - Sokolov, I.

N1 - Funding Information: Support of this work from the National Science Foundation is acknowledged by I.S. (CMMI 1435655 , CMMI 1937373 ) and from the National Institutes of Health by L.Z. ( R01 CA103846 , P01 CA163222 ). Publisher Copyright: © 2020

PY - 2020/7

Y1 - 2020/7

N2 - Despite sharing oncogenetic mutations, only a small number of cells within a given tissue will undergo malignant transformation. Biochemical and physical factors responsible for this cancer-initiation process are not well understood. Here we study biophysical differences of pre-melanoma and melanoma cells in a BRAFV600E/P53 zebrafish model. The AFM indentation technique was used to study the cancer-initiating cells while the surrounding melanocytes were the control. We observed a statistically significant decrease in the modulus of elasticity (the effective Young's modulus) of cancer-initiating cells compared to the surrounding melanocytes. No significant differences in the pericellular coat surrounding cells were observed. These results contribute to a better understanding of the factors responsible for the initiation of cancer.

AB - Despite sharing oncogenetic mutations, only a small number of cells within a given tissue will undergo malignant transformation. Biochemical and physical factors responsible for this cancer-initiation process are not well understood. Here we study biophysical differences of pre-melanoma and melanoma cells in a BRAFV600E/P53 zebrafish model. The AFM indentation technique was used to study the cancer-initiating cells while the surrounding melanocytes were the control. We observed a statistically significant decrease in the modulus of elasticity (the effective Young's modulus) of cancer-initiating cells compared to the surrounding melanocytes. No significant differences in the pericellular coat surrounding cells were observed. These results contribute to a better understanding of the factors responsible for the initiation of cancer.

KW - Atomic force microscopy

KW - Cell mechanics

KW - Initiation of cancer

KW - Pericellular coat

KW - Zebrafish

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JO - Journal of the Mechanical Behavior of Biomedical Materials

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M1 - 103746

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Difference in biophysical properties of cancer-initiating cells in melanoma mutated zebrafish

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Keywords

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