Diagnostic assays for identification of microorganisms and antimicrobial resistance determinants directly from positive blood culture broth

Morgan A. Pence; Erin McElvania TeKippe; Carey Ann D. Burnham

doi:10.1016/j.cll.2013.03.010

Diagnostic assays for identification of microorganisms and antimicrobial resistance determinants directly from positive blood culture broth

Morgan A. Pence, Erin McElvania TeKippe, Carey Ann D. Burnham

Research output: Contribution to journal › Review article › peer-review

27 Scopus citations

Abstract

The detection of blood stream infections is one of the most important functions of the clinical microbiology laboratory. Sepsis is a clinical emergency, and mortality increases if commencement of appropriate antimicrobial therapy is delayed. Automated blood culture systems are the most sensitive approach for detection of the causative agent of sepsis. Several laboratory methods have been developed to expedite identification of organisms directly from positive blood culture broth. The principle and analytical performance characteristics of these methods are described in this review.

Original language	English
Pages (from-to)	651-684
Number of pages	34
Journal	Clinics in Laboratory Medicine
Volume	33
Issue number	3
DOIs	https://doi.org/10.1016/j.cll.2013.03.010
State	Published - Sep 2013

Keywords

Bacteremia
Blood culture
MRSA
Molecular diagnostics
Rapid pathogen detection
Staphylococcus aureus

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10.1016/j.cll.2013.03.010

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@article{f54661d72c384bbcb699e8475e67a638,

title = "Diagnostic assays for identification of microorganisms and antimicrobial resistance determinants directly from positive blood culture broth",

abstract = "The detection of blood stream infections is one of the most important functions of the clinical microbiology laboratory. Sepsis is a clinical emergency, and mortality increases if commencement of appropriate antimicrobial therapy is delayed. Automated blood culture systems are the most sensitive approach for detection of the causative agent of sepsis. Several laboratory methods have been developed to expedite identification of organisms directly from positive blood culture broth. The principle and analytical performance characteristics of these methods are described in this review.",

keywords = "Bacteremia, Blood culture, MRSA, Molecular diagnostics, Rapid pathogen detection, Staphylococcus aureus",

author = "Pence, {Morgan A.} and {McElvania TeKippe}, Erin and Burnham, {Carey Ann D.}",

note = "Funding Information: Disclosures: C.A. Burnham has received research funding from Cepheid , Accelr8 , bioM{\'e}rieux and Luminex Molecular Diagnostics . The other authors have no potential conflicts of interest to report. ",

year = "2013",

month = sep,

doi = "10.1016/j.cll.2013.03.010",

language = "English",

volume = "33",

pages = "651--684",

journal = "Clinics in Laboratory Medicine",

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T1 - Diagnostic assays for identification of microorganisms and antimicrobial resistance determinants directly from positive blood culture broth

AU - Pence, Morgan A.

AU - McElvania TeKippe, Erin

AU - Burnham, Carey Ann D.

N1 - Funding Information: Disclosures: C.A. Burnham has received research funding from Cepheid , Accelr8 , bioMérieux and Luminex Molecular Diagnostics . The other authors have no potential conflicts of interest to report.

PY - 2013/9

Y1 - 2013/9

N2 - The detection of blood stream infections is one of the most important functions of the clinical microbiology laboratory. Sepsis is a clinical emergency, and mortality increases if commencement of appropriate antimicrobial therapy is delayed. Automated blood culture systems are the most sensitive approach for detection of the causative agent of sepsis. Several laboratory methods have been developed to expedite identification of organisms directly from positive blood culture broth. The principle and analytical performance characteristics of these methods are described in this review.

AB - The detection of blood stream infections is one of the most important functions of the clinical microbiology laboratory. Sepsis is a clinical emergency, and mortality increases if commencement of appropriate antimicrobial therapy is delayed. Automated blood culture systems are the most sensitive approach for detection of the causative agent of sepsis. Several laboratory methods have been developed to expedite identification of organisms directly from positive blood culture broth. The principle and analytical performance characteristics of these methods are described in this review.

KW - Bacteremia

KW - Blood culture

KW - MRSA

KW - Molecular diagnostics

KW - Rapid pathogen detection

KW - Staphylococcus aureus

UR - http://www.scopus.com/inward/record.url?scp=84881517805&partnerID=8YFLogxK

U2 - 10.1016/j.cll.2013.03.010

DO - 10.1016/j.cll.2013.03.010

M3 - Review article

C2 - 23931843

AN - SCOPUS:84881517805

SN - 0272-2712

VL - 33

SP - 651

EP - 684

JO - Clinics in Laboratory Medicine

JF - Clinics in Laboratory Medicine

IS - 3

ER -

Diagnostic assays for identification of microorganisms and antimicrobial resistance determinants directly from positive blood culture broth

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Keywords

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