Diagnosis of Capnocytophaga canimorsus sepsis by whole-genome next-generation sequencing

Maria K. Abril; Adam S. Barnett; Kara Wegermann; Eric Fountain; Andrew Strand; Benjamin M. Heyman; Britton A. Blough; Aparna C. Swaminathan; Batu Sharma-Kuinkel; Felicia Ruffin; Barbara D. Alexander; Chad M. McCall; Sylvia F. Costa; Murat O. Arcasoy; David K. Hong; Timothy A. Blauwkamp; Michael Kertesz; Vance G. Fowler; Bryan D. Kraft

doi:10.1093/ofid/ofw144

Diagnosis of Capnocytophaga canimorsus sepsis by whole-genome next-generation sequencing

Maria K. Abril, Adam S. Barnett, Kara Wegermann, Eric Fountain, Andrew Strand, Benjamin M. Heyman, Britton A. Blough, Aparna C. Swaminathan, Batu Sharma-Kuinkel, Felicia Ruffin, Barbara D. Alexander, Chad M. McCall, Sylvia F. Costa, Murat O. Arcasoy, David K. Hong, Timothy A. Blauwkamp, Michael Kertesz, Vance G. Fowler, Bryan D. Kraft

Division of Pulmonary & Critical Care Medicine

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

We report the case of a 60-year-old man with septic shock due to Capnocytophaga canimorsus that was diagnosed in 24 hours by a novel whole-genome next-generation sequencing assay. This technology shows great promise in identifying fastidious pathogens, and, if validated, it has profound implications for infectious disease diagnosis.

Original language	English
Journal	Open Forum Infectious Diseases
Volume	3
Issue number	3
DOIs	https://doi.org/10.1093/ofid/ofw144
State	Published - May 1 2016

Keywords

High-throughput nucleotide sequencing
Microbiological techniques
Sepsis/diagnosis

Access to Document

10.1093/ofid/ofw144

Cite this

Abril, M. K., Barnett, A. S., Wegermann, K., Fountain, E., Strand, A., Heyman, B. M., Blough, B. A., Swaminathan, A. C., Sharma-Kuinkel, B., Ruffin, F., Alexander, B. D., McCall, C. M., Costa, S. F., Arcasoy, M. O., Hong, D. K., Blauwkamp, T. A., Kertesz, M., Fowler, V. G., & Kraft, B. D. (2016). Diagnosis of Capnocytophaga canimorsus sepsis by whole-genome next-generation sequencing. Open Forum Infectious Diseases, 3(3). https://doi.org/10.1093/ofid/ofw144

@article{c8f662fcd4ff41fdbf6bd935825b2872,

title = "Diagnosis of Capnocytophaga canimorsus sepsis by whole-genome next-generation sequencing",

abstract = "We report the case of a 60-year-old man with septic shock due to Capnocytophaga canimorsus that was diagnosed in 24 hours by a novel whole-genome next-generation sequencing assay. This technology shows great promise in identifying fastidious pathogens, and, if validated, it has profound implications for infectious disease diagnosis.",

keywords = "High-throughput nucleotide sequencing, Microbiological techniques, Sepsis/diagnosis",

author = "Abril, {Maria K.} and Barnett, {Adam S.} and Kara Wegermann and Eric Fountain and Andrew Strand and Heyman, {Benjamin M.} and Blough, {Britton A.} and Swaminathan, {Aparna C.} and Batu Sharma-Kuinkel and Felicia Ruffin and Alexander, {Barbara D.} and McCall, {Chad M.} and Costa, {Sylvia F.} and Arcasoy, {Murat O.} and Hong, {David K.} and Blauwkamp, {Timothy A.} and Michael Kertesz and Fowler, {Vance G.} and Kraft, {Bryan D.}",

note = "Publisher Copyright: {\textcopyright} The Author 2016.",

year = "2016",

month = may,

day = "1",

doi = "10.1093/ofid/ofw144",

language = "English",

volume = "3",

journal = "Open Forum Infectious Diseases",

issn = "2328-8957",

number = "3",

}

Abril, MK, Barnett, AS, Wegermann, K, Fountain, E, Strand, A, Heyman, BM, Blough, BA, Swaminathan, AC, Sharma-Kuinkel, B, Ruffin, F, Alexander, BD, McCall, CM, Costa, SF, Arcasoy, MO, Hong, DK, Blauwkamp, TA, Kertesz, M, Fowler, VG & Kraft, BD 2016, 'Diagnosis of Capnocytophaga canimorsus sepsis by whole-genome next-generation sequencing', Open Forum Infectious Diseases, vol. 3, no. 3. https://doi.org/10.1093/ofid/ofw144

TY - JOUR

T1 - Diagnosis of Capnocytophaga canimorsus sepsis by whole-genome next-generation sequencing

AU - Abril, Maria K.

AU - Barnett, Adam S.

AU - Wegermann, Kara

AU - Fountain, Eric

AU - Strand, Andrew

AU - Heyman, Benjamin M.

AU - Blough, Britton A.

AU - Swaminathan, Aparna C.

AU - Sharma-Kuinkel, Batu

AU - Ruffin, Felicia

AU - Alexander, Barbara D.

AU - McCall, Chad M.

AU - Costa, Sylvia F.

AU - Arcasoy, Murat O.

AU - Hong, David K.

AU - Blauwkamp, Timothy A.

AU - Kertesz, Michael

AU - Fowler, Vance G.

AU - Kraft, Bryan D.

PY - 2016/5/1

Y1 - 2016/5/1

N2 - We report the case of a 60-year-old man with septic shock due to Capnocytophaga canimorsus that was diagnosed in 24 hours by a novel whole-genome next-generation sequencing assay. This technology shows great promise in identifying fastidious pathogens, and, if validated, it has profound implications for infectious disease diagnosis.

AB - We report the case of a 60-year-old man with septic shock due to Capnocytophaga canimorsus that was diagnosed in 24 hours by a novel whole-genome next-generation sequencing assay. This technology shows great promise in identifying fastidious pathogens, and, if validated, it has profound implications for infectious disease diagnosis.

KW - High-throughput nucleotide sequencing

KW - Microbiological techniques

KW - Sepsis/diagnosis

UR - http://www.scopus.com/inward/record.url?scp=85015983306&partnerID=8YFLogxK

U2 - 10.1093/ofid/ofw144

DO - 10.1093/ofid/ofw144

M3 - Article

AN - SCOPUS:85015983306

SN - 2328-8957

VL - 3

JO - Open Forum Infectious Diseases

JF - Open Forum Infectious Diseases

IS - 3

ER -

Diagnosis of Capnocytophaga canimorsus sepsis by whole-genome next-generation sequencing

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this