Developmentally regulated rearrangement and expression of genes encoding the T cell receptor-T3 complex

A. J. Furley; S. Mizutani; K. Weilbaecher; H. S. Dhaliwal; A. M. Ford; L. C. Chan; H. V. Molgaard; B. Toyonaga; T. Mak; P. van den Elsen; D. Gold; C. Terhorst; M. F. Greaves

doi:10.1016/0092-8674(86)90861-5

Developmentally regulated rearrangement and expression of genes encoding the T cell receptor-T3 complex

A. J. Furley, S. Mizutani, K. Weilbaecher, H. S. Dhaliwal, A. M. Ford, L. C. Chan, H. V. Molgaard, B. Toyonaga, T. Mak, P. van den Elsen, D. Gold, C. Terhorst, M. F. Greaves

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Abstract

Human leukemic cells corresponding to the earliest identifiable stages of intrathymic T cell differentiation lack cell surface expression of the T cell receptor(TCRα/β)-T3 complex but transcribe TCRβ mRNA from either germ-line configuration ( 1 13) or partially (DJ) or fully (VDJ) rearranged ( 12 13) genes. These cells do not produce TCRα mRNA, but do contain T3δ and T3ε{lunate} mRNA and accumulate T3 polypeptides, primarily in the perinuclear envelope. Equivalent normal T cells isolated from thymus have a predominantly germ-line configuration of TCRβ but contain intracellular T3 proteins. T3 gene expression is therefore a very early event in T cell differentiation. TCRα chain production appears to be the limiting maturation-linked event in the transport, assembly, and cell surface membrane insertion of the TCRα β-T3 complex.

Original language	English
Pages (from-to)	75-87
Number of pages	13
Journal	Cell
Volume	46
Issue number	1
DOIs	https://doi.org/10.1016/0092-8674(86)90861-5
State	Published - Jul 4 1986

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@article{94453160aea445349e6f620dc71a42e0,

title = "Developmentally regulated rearrangement and expression of genes encoding the T cell receptor-T3 complex",

abstract = "Human leukemic cells corresponding to the earliest identifiable stages of intrathymic T cell differentiation lack cell surface expression of the T cell receptor(TCRα/β)-T3 complex but transcribe TCRβ mRNA from either germ-line configuration ( 1 13) or partially (DJ) or fully (VDJ) rearranged ( 12 13) genes. These cells do not produce TCRα mRNA, but do contain T3δ and T3ε{lunate} mRNA and accumulate T3 polypeptides, primarily in the perinuclear envelope. Equivalent normal T cells isolated from thymus have a predominantly germ-line configuration of TCRβ but contain intracellular T3 proteins. T3 gene expression is therefore a very early event in T cell differentiation. TCRα chain production appears to be the limiting maturation-linked event in the transport, assembly, and cell surface membrane insertion of the TCRα β-T3 complex.",

author = "Furley, {A. J.} and S. Mizutani and K. Weilbaecher and Dhaliwal, {H. S.} and Ford, {A. M.} and Chan, {L. C.} and Molgaard, {H. V.} and B. Toyonaga and T. Mak and {van den Elsen}, P. and D. Gold and C. Terhorst and Greaves, {M. F.}",

note = "Funding Information: This work was supported by the Leukaemia Research Fund of Great Britain and NIH grant (to C. T.) number Al176.51. A. J. W. Furley receives an Imperial Cancer Research Fund bursarship. We are grateful to Sue Pegram and Lynn Altass for their excellent technical assistance, to Dr. L. Wiedemann for critical comments, to numerous colleagues who have supplied monoclonal antibodies and clinical material, and to Geraldine Parkins and Jackie Needham for help in preparation of the manuscript.",

year = "1986",

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doi = "10.1016/0092-8674(86)90861-5",

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T1 - Developmentally regulated rearrangement and expression of genes encoding the T cell receptor-T3 complex

AU - Furley, A. J.

AU - Mizutani, S.

AU - Weilbaecher, K.

AU - Dhaliwal, H. S.

AU - Ford, A. M.

AU - Chan, L. C.

AU - Molgaard, H. V.

AU - Toyonaga, B.

AU - Mak, T.

AU - van den Elsen, P.

AU - Gold, D.

AU - Terhorst, C.

AU - Greaves, M. F.

N1 - Funding Information: This work was supported by the Leukaemia Research Fund of Great Britain and NIH grant (to C. T.) number Al176.51. A. J. W. Furley receives an Imperial Cancer Research Fund bursarship. We are grateful to Sue Pegram and Lynn Altass for their excellent technical assistance, to Dr. L. Wiedemann for critical comments, to numerous colleagues who have supplied monoclonal antibodies and clinical material, and to Geraldine Parkins and Jackie Needham for help in preparation of the manuscript.

PY - 1986/7/4

Y1 - 1986/7/4

N2 - Human leukemic cells corresponding to the earliest identifiable stages of intrathymic T cell differentiation lack cell surface expression of the T cell receptor(TCRα/β)-T3 complex but transcribe TCRβ mRNA from either germ-line configuration ( 1 13) or partially (DJ) or fully (VDJ) rearranged ( 12 13) genes. These cells do not produce TCRα mRNA, but do contain T3δ and T3ε{lunate} mRNA and accumulate T3 polypeptides, primarily in the perinuclear envelope. Equivalent normal T cells isolated from thymus have a predominantly germ-line configuration of TCRβ but contain intracellular T3 proteins. T3 gene expression is therefore a very early event in T cell differentiation. TCRα chain production appears to be the limiting maturation-linked event in the transport, assembly, and cell surface membrane insertion of the TCRα β-T3 complex.

AB - Human leukemic cells corresponding to the earliest identifiable stages of intrathymic T cell differentiation lack cell surface expression of the T cell receptor(TCRα/β)-T3 complex but transcribe TCRβ mRNA from either germ-line configuration ( 1 13) or partially (DJ) or fully (VDJ) rearranged ( 12 13) genes. These cells do not produce TCRα mRNA, but do contain T3δ and T3ε{lunate} mRNA and accumulate T3 polypeptides, primarily in the perinuclear envelope. Equivalent normal T cells isolated from thymus have a predominantly germ-line configuration of TCRβ but contain intracellular T3 proteins. T3 gene expression is therefore a very early event in T cell differentiation. TCRα chain production appears to be the limiting maturation-linked event in the transport, assembly, and cell surface membrane insertion of the TCRα β-T3 complex.

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Developmentally regulated rearrangement and expression of genes encoding the T cell receptor-T3 complex

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