Developmental transitions in the myosin heavy chain phenotype of human respiratory muscle

Janet S. Lloyd, Beverly S. Brozanski, Monica Daood, Jon F. Watchko

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We studied the expression of myosin heavy chain (MHC) isoforms in the costal diaphragm (DIA) and the genioglossus (GG) muscles from 16 to 42 weeks gestation in the human using Western blotting techniques. Embryonic/neonatal MHC (MHCemb/neo) was the predominant isoform expressed in the DIA and GG at 16-24 weeks gestation. Subsequently, MHCemb/neo expression declined and the expression of MHCslow and MHC2A increased. At term, the DIA MHC phenotype was a composite of MHCemb/neo (15% of the total MHC complement), MHCslow (32%), MHC2A (47%), and MHC2B (6%); whereas, the GG was largely comprised of MHC2A (74%). We conclude that human DIA and GG demonstrate temporally dependent changes in MHC expression during gestation- and muscle-specific MHC phenotypes as they approach term.

Original languageEnglish
Pages (from-to)67-75
Number of pages9
JournalNeonatology
Volume69
Issue number2
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Keywords

  • Diaphragm
  • Electrophoresis
  • Genioglossus
  • Myosin heavy chain

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