Developmental transitions in the myosin heavy chain phenotype of human respiratory muscle

Janet S. Lloyd; Beverly S. Brozanski; Monica Daood; Jon F. Watchko

doi:10.1159/000244280

Developmental transitions in the myosin heavy chain phenotype of human respiratory muscle

Janet S. Lloyd, Beverly S. Brozanski, Monica Daood, Jon F. Watchko

Division of Newborn Medicine

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We studied the expression of myosin heavy chain (MHC) isoforms in the costal diaphragm (DIA) and the genioglossus (GG) muscles from 16 to 42 weeks gestation in the human using Western blotting techniques. Embryonic/neonatal MHC (MHC_emb/_neo) was the predominant isoform expressed in the DIA and GG at 16-24 weeks gestation. Subsequently, MHC_emb/_neo expression declined and the expression of MHC_slow and MHC_2A increased. At term, the DIA MHC phenotype was a composite of MHC_emb/_neo (15% of the total MHC complement), MHC_slow (32%), MHC_2A (47%), and MHC_2B (6%); whereas, the GG was largely comprised of MHC_2A (74%). We conclude that human DIA and GG demonstrate temporally dependent changes in MHC expression during gestation- and muscle-specific MHC phenotypes as they approach term.

Original language	English
Pages (from-to)	67-75
Number of pages	9
Journal	Neonatology
Volume	69
Issue number	2
DOIs	https://doi.org/10.1159/000244280
State	Published - Jan 1 1996

Keywords

Diaphragm
Electrophoresis
Genioglossus
Myosin heavy chain

Access to Document

10.1159/000244280

Cite this

@article{66f6b67d72894028971033751553db08,

title = "Developmental transitions in the myosin heavy chain phenotype of human respiratory muscle",

abstract = "We studied the expression of myosin heavy chain (MHC) isoforms in the costal diaphragm (DIA) and the genioglossus (GG) muscles from 16 to 42 weeks gestation in the human using Western blotting techniques. Embryonic/neonatal MHC (MHCemb/neo) was the predominant isoform expressed in the DIA and GG at 16-24 weeks gestation. Subsequently, MHCemb/neo expression declined and the expression of MHCslow and MHC2A increased. At term, the DIA MHC phenotype was a composite of MHCemb/neo (15% of the total MHC complement), MHCslow (32%), MHC2A (47%), and MHC2B (6%); whereas, the GG was largely comprised of MHC2A (74%). We conclude that human DIA and GG demonstrate temporally dependent changes in MHC expression during gestation- and muscle-specific MHC phenotypes as they approach term.",

keywords = "Diaphragm, Electrophoresis, Genioglossus, Myosin heavy chain",

author = "Lloyd, {Janet S.} and Brozanski, {Beverly S.} and Monica Daood and Watchko, {Jon F.}",

year = "1996",

month = jan,

day = "1",

doi = "10.1159/000244280",

language = "English",

volume = "69",

pages = "67--75",

journal = "Neonatology",

issn = "1661-7800",

number = "2",

}

TY - JOUR

T1 - Developmental transitions in the myosin heavy chain phenotype of human respiratory muscle

AU - Lloyd, Janet S.

AU - Brozanski, Beverly S.

AU - Daood, Monica

AU - Watchko, Jon F.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - We studied the expression of myosin heavy chain (MHC) isoforms in the costal diaphragm (DIA) and the genioglossus (GG) muscles from 16 to 42 weeks gestation in the human using Western blotting techniques. Embryonic/neonatal MHC (MHCemb/neo) was the predominant isoform expressed in the DIA and GG at 16-24 weeks gestation. Subsequently, MHCemb/neo expression declined and the expression of MHCslow and MHC2A increased. At term, the DIA MHC phenotype was a composite of MHCemb/neo (15% of the total MHC complement), MHCslow (32%), MHC2A (47%), and MHC2B (6%); whereas, the GG was largely comprised of MHC2A (74%). We conclude that human DIA and GG demonstrate temporally dependent changes in MHC expression during gestation- and muscle-specific MHC phenotypes as they approach term.

AB - We studied the expression of myosin heavy chain (MHC) isoforms in the costal diaphragm (DIA) and the genioglossus (GG) muscles from 16 to 42 weeks gestation in the human using Western blotting techniques. Embryonic/neonatal MHC (MHCemb/neo) was the predominant isoform expressed in the DIA and GG at 16-24 weeks gestation. Subsequently, MHCemb/neo expression declined and the expression of MHCslow and MHC2A increased. At term, the DIA MHC phenotype was a composite of MHCemb/neo (15% of the total MHC complement), MHCslow (32%), MHC2A (47%), and MHC2B (6%); whereas, the GG was largely comprised of MHC2A (74%). We conclude that human DIA and GG demonstrate temporally dependent changes in MHC expression during gestation- and muscle-specific MHC phenotypes as they approach term.

KW - Diaphragm

KW - Electrophoresis

KW - Genioglossus

KW - Myosin heavy chain

UR - http://www.scopus.com/inward/record.url?scp=0029970606&partnerID=8YFLogxK

U2 - 10.1159/000244280

DO - 10.1159/000244280

M3 - Article

C2 - 8713651

AN - SCOPUS:0029970606

SN - 1661-7800

VL - 69

SP - 67

EP - 75

JO - Neonatology

JF - Neonatology

IS - 2

ER -

Developmental transitions in the myosin heavy chain phenotype of human respiratory muscle

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this