TY - JOUR
T1 - Developmental changes in myocardial B cells mirror changes in B cells associated with different organs
AU - Rocha-Resende, Cibele
AU - Yang, Wei
AU - Li, Wenjun
AU - Kreisel, Daniel
AU - Adamo, Luigi
AU - Mann, Douglas L.
N1 - Funding Information:
This study was supported by research funds from the NIH (R01 HL-58081, HL-73017-0, HL-089543-01, HL 111094, R00 HL138163, T32 HL007081, S10OD020136, and 1K08HL145108-01A1), institutional funds from the Center for Cardiovascular Research at Washington University School of Medicine, and a pilot grant from the McDonnell Genome Institute at Washington University. The authors acknowledge Erica Lantelme and the Washington University Flow Cytometry & Fluorescence Activated Cell Sorting Core for assistance with FACS analysis and cell sorting. The authors acknowledge also Dennis Oakley and the WUCCI for assistance with confocal imaging.
Funding Information:
This study was supported by research funds from the NIH (R01 HL-58081, HL-73017-0, HL-089543-01, HL 111094, R00 HL138163, T32 HL007081, S10OD020136, and 1K08HL145108-01A1), institutional funds from the Center for Cardiovascular Research at Washington University School of Medicine, and a pilot grant from the McDonnell Genome Institute at Washington University. The authors acknowledge Erica Lantelme and the Washington University Flow Cytometry & Fluorescence Activated Cell Sorting Core for assistance with FACS analysis and cell sorting. The authors acknowledge also Dennis Oakley and the WUCCI for assistance with confocal imaging. Address correspondence to: Luigi Adamo or Douglas L. Mann, Division of Cardiology, 660 S. Euclid Avenue, Campus Box 8086, St. Louis, Missouri 63110, USA. Phone: 314.273.7987; Email: ladamo@ wustl.edu (LA). Phone: 314.362.8908; Email: dmann@wustl.edu (DLM).
Publisher Copyright:
Copyright: © 2020, Rocha-Resende et al. This is an open access article published under the terms of the Creative Commons Attribution 4.0 International License.
PY - 2020/8/20
Y1 - 2020/8/20
N2 - The naive heart harbors a population of intravascular B cells that make close contact with the cardiac microvasculature. However, the timing of their appearance and their organ specificity remain unknown. To address this knowledge gap, we performed a systematic analysis of B cells isolated from the myocardium and other organs, from embryonic life to adulthood. We found that the phenotype of myocardial B cells changed dynamically during development. While neonatal heart B cells were mostly CD11b+ and CD11b- CD21-CD23-, adult B cells were predominantly CD11b-CD21+CD23+. Histological analysis and intravital microscopy of lung and liver showed that organ-associated B cells in contact with the microvascular endothelium were not specific to the heart. Flow cytometric analysis of perfused hearts, livers, lungs, and spleen showed that the dynamic changes in B cell subpopulations observed in the heart during development mirrored changes observed in the other organs. Single cell RNA sequencing (scRNAseq) analysis of B cells showed that myocardial B cells were part of a larger population of organ-associated B cells that had a distinct transcriptional profile. These findings broaden our understanding of the biology of myocardial-associated B cells and suggest that current models of the dynamics of naive B cells during development are incomplete.
AB - The naive heart harbors a population of intravascular B cells that make close contact with the cardiac microvasculature. However, the timing of their appearance and their organ specificity remain unknown. To address this knowledge gap, we performed a systematic analysis of B cells isolated from the myocardium and other organs, from embryonic life to adulthood. We found that the phenotype of myocardial B cells changed dynamically during development. While neonatal heart B cells were mostly CD11b+ and CD11b- CD21-CD23-, adult B cells were predominantly CD11b-CD21+CD23+. Histological analysis and intravital microscopy of lung and liver showed that organ-associated B cells in contact with the microvascular endothelium were not specific to the heart. Flow cytometric analysis of perfused hearts, livers, lungs, and spleen showed that the dynamic changes in B cell subpopulations observed in the heart during development mirrored changes observed in the other organs. Single cell RNA sequencing (scRNAseq) analysis of B cells showed that myocardial B cells were part of a larger population of organ-associated B cells that had a distinct transcriptional profile. These findings broaden our understanding of the biology of myocardial-associated B cells and suggest that current models of the dynamics of naive B cells during development are incomplete.
UR - http://www.scopus.com/inward/record.url?scp=85089768465&partnerID=8YFLogxK
U2 - 10.1172/jci.insight.139377
DO - 10.1172/jci.insight.139377
M3 - Article
C2 - 32663200
AN - SCOPUS:85089768465
SN - 2379-3708
VL - 5
JO - JCI insight
JF - JCI insight
IS - 16
M1 - e139377
ER -