TY - JOUR
T1 - Developmental alteration in GABAA receptor structure and physiological properties in cultured cerebellar granule neurons
AU - Mathews, Gregory C.
AU - Bolos-Sy, Annabel M.
AU - Holland, Katherine D.
AU - Isenberg, Keith E.
AU - Covey, Douglas F.
AU - Ferrendelli, James A.
AU - Rothman, Steven M.
N1 - Funding Information:
This work was supported by National Institutes of Health grants NS14.834 and 5T32CM07805 and the Seay Fellowship. We are gratefuItoDr.C.Fraserforthea6andy2Lclones,Dr.P.Malherbe for the ~32 clone, and Dr. A. Tobin for the al clone. We thank Joseph B. Tucker for technical assistance. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC Section 1734 solely to indicate fact.
PY - 1994/7
Y1 - 1994/7
N2 - Although we now have extensive knowledge about the GABAA receptor subunits determining benzodiazepine modulation of channel function, little is known about subunits influencing other modulatory sites on the GABAA receptor-chloride channel complex. We have identified a developmental change in subunit composition of the GABAA receptor in cultured cerebellar granule neurons that eliminates benzodiazepine-mediated enhancement of GABA responses and alters modulation by a substituted γ-butyrolactone. Based on data from sequential PCR experiments, we mimicked the functional properties of early and mature receptors with heterologous expression of specific subunit combinations. This report describes one of the most extensive cell- and site-specific developmental changes for an ion channel seen to date.
AB - Although we now have extensive knowledge about the GABAA receptor subunits determining benzodiazepine modulation of channel function, little is known about subunits influencing other modulatory sites on the GABAA receptor-chloride channel complex. We have identified a developmental change in subunit composition of the GABAA receptor in cultured cerebellar granule neurons that eliminates benzodiazepine-mediated enhancement of GABA responses and alters modulation by a substituted γ-butyrolactone. Based on data from sequential PCR experiments, we mimicked the functional properties of early and mature receptors with heterologous expression of specific subunit combinations. This report describes one of the most extensive cell- and site-specific developmental changes for an ion channel seen to date.
UR - http://www.scopus.com/inward/record.url?scp=0028169941&partnerID=8YFLogxK
U2 - 10.1016/0896-6273(94)90465-0
DO - 10.1016/0896-6273(94)90465-0
M3 - Article
C2 - 8043274
AN - SCOPUS:0028169941
SN - 0896-6273
VL - 13
SP - 149
EP - 158
JO - Neuron
JF - Neuron
IS - 1
ER -