Developmental alteration in GABAA receptor structure and physiological properties in cultured cerebellar granule neurons

Gregory C. Mathews, Annabel M. Bolos-Sy, Katherine D. Holland, Keith E. Isenberg, Douglas F. Covey, James A. Ferrendelli, Steven M. Rothman

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

Although we now have extensive knowledge about the GABAA receptor subunits determining benzodiazepine modulation of channel function, little is known about subunits influencing other modulatory sites on the GABAA receptor-chloride channel complex. We have identified a developmental change in subunit composition of the GABAA receptor in cultured cerebellar granule neurons that eliminates benzodiazepine-mediated enhancement of GABA responses and alters modulation by a substituted γ-butyrolactone. Based on data from sequential PCR experiments, we mimicked the functional properties of early and mature receptors with heterologous expression of specific subunit combinations. This report describes one of the most extensive cell- and site-specific developmental changes for an ion channel seen to date.

Original languageEnglish
Pages (from-to)149-158
Number of pages10
JournalNeuron
Volume13
Issue number1
DOIs
StatePublished - Jul 1994

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