TY - JOUR
T1 - Development of the fast, transient outward K+ current in embryonic sympathetic neurones
AU - Nerbonne, Jeanne M.
AU - Gurney, Alison M.
AU - Rayburn, Helen B.
N1 - Funding Information:
supported by the American Heart Association, Los Angeles Affiliate (Postdoctoral Fellowships to J.M.N. and A.M.G.) and National Office (Established Investigator Award to J.M.N.), by the Systems Development Foundation (Grant 238 to J. Pine) and by the National Institutes of Health (Grant GM-29836 to H.A. Lester).
PY - 1986/7/16
Y1 - 1986/7/16
N2 - Voltage-activated outward potassium (K+) currents in developing sympathetic neurones, dissociated from the rat superior cervical ganglion (SCG), were studied using the whole-cell patch clamp recording technique. In voltage-clamped neonatal SCG cells, two voltage-dependent K+ currents were measured: the fast, transient K+ current, IA; and, the slower activating, non-inactivating delayed rectifier, IK. Only IK, however, appeared to be present in SCG neurones isolated from early embryonic (E14.5-16.5) rat pups: IA was not observed in these cells. When these embryonic neurones were maintained in cell culture, IA developed over a time course (≈ 4-6 days) similar to that seen in vivo. IA, therefore, which appears to facilitate the fast repolarization phase of the action potential in rat SCG neurones, is the last voltage-activated current to develop in these cells.
AB - Voltage-activated outward potassium (K+) currents in developing sympathetic neurones, dissociated from the rat superior cervical ganglion (SCG), were studied using the whole-cell patch clamp recording technique. In voltage-clamped neonatal SCG cells, two voltage-dependent K+ currents were measured: the fast, transient K+ current, IA; and, the slower activating, non-inactivating delayed rectifier, IK. Only IK, however, appeared to be present in SCG neurones isolated from early embryonic (E14.5-16.5) rat pups: IA was not observed in these cells. When these embryonic neurones were maintained in cell culture, IA developed over a time course (≈ 4-6 days) similar to that seen in vivo. IA, therefore, which appears to facilitate the fast repolarization phase of the action potential in rat SCG neurones, is the last voltage-activated current to develop in these cells.
KW - I
KW - development
KW - sympathetic neuron
KW - transient outward current
KW - whole-cell voltage clamp
UR - http://www.scopus.com/inward/record.url?scp=0022502942&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(86)90306-9
DO - 10.1016/0006-8993(86)90306-9
M3 - Article
C2 - 2427156
AN - SCOPUS:0022502942
SN - 0006-8993
VL - 378
SP - 197
EP - 202
JO - Brain Research
JF - Brain Research
IS - 1
ER -