Development of nasal allergen challenge with cockroach in children with asthma

the National Institute of Allergy and Infectious Diseases Inner City Asthma Consortium

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Background: Nasal allergen challenge (NAC) could be a means to assess indication and/or an outcome of allergen-specific therapies, particularly for perennial allergens. NACs are not commonly conducted in children with asthma, and cockroach NACs are not well established. This study's objective was to identify a range of German cockroach extract doses that induce nasal symptoms and to assess the safety of cockroach NAC in children with asthma. Methods: Ten adults (18-37 years) followed by 25 children (8-14 years) with well-controlled, persistent asthma and cockroach sensitization underwent NAC with diluent followed by up to 8 escalating doses of cockroach extract (0.00381-11.9 µg/mL Bla g 1). NAC outcome was determined by Total Nasal Symptom Score (TNSS) and/or sneeze score. Cockroach allergen–induced T-cell activation and IL-5 production were measured in peripheral blood mononuclear cells. Results: 67% (6/9) of adults and 68% (17/25) of children had a positive NAC at a median response dose of 0.120 µg/mL [IQR 0.0380-0.379 µg/mL] of Bla g 1. Additionally, three children responded to diluent alone and did not receive any cockroach extract. Overall, 32% (11/34) were positive with sneezes alone, 15% (5/34) with TNSS alone, and 21% (7/34) with both criteria. At baseline, NAC responders had higher cockroach-specific IgE (P =.03), lower cockroach-specific IgG/IgE ratios (children, P =.002), and increased cockroach-specific IL-5–producing T lymphocytes (P =.045). The NAC was well tolerated. Conclusion: We report the methodology of NAC development for children with persistent asthma and cockroach sensitization. This NAC could be considered a tool to confirm clinically relevant sensitization and to assess responses in therapeutic studies.

Original languageEnglish
Pages (from-to)971-979
Number of pages9
JournalPediatric Allergy and Immunology
Issue number5
StatePublished - Jul 2021


  • allergic rhinitis
  • asthma
  • children
  • cockroach allergy
  • inner city
  • nasal allergen challenge


Dive into the research topics of 'Development of nasal allergen challenge with cockroach in children with asthma'. Together they form a unique fingerprint.

Cite this