Development of Monoclonal Antibodies to Detect for SARS-CoV-2 Proteins

Nawneet Mishra, Joan Teyra, Ruth Mabel Boytz, Shane Miersch, Trudy N. Merritt, Lia Cardarelli, Maryna Gorelik, Filip Mihalic, Per Jemth, Robert A. Davey, Sachdev S. Sidhu, Daisy W. Leung, Gaya K. Amarasinghe

Research output: Contribution to journalArticlepeer-review

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 infection has impacted the world economy and healthcare infrastructure. Key reagents with high specificity to SARS-CoV-2 proteins are currently lacking, which limits our ability to understand the pathophysiology of SARS-CoV-2 infections. To address this need, we initiated a series of studies to generate and develop highly specific antibodies against proteins from SARS-CoV-2 using an antibody engineering platform. These efforts resulted in 18 monoclonal antibodies against nine SARS-CoV-2 proteins. Here we report the characterization of several antibodies, including those that recognize Nsp1, Nsp8, Nsp12, and Orf3b viral proteins. Our validation studies included evaluation for use of antibodies in ELISA, western blots, and immunofluorescence assays (IFA). We expect that availability of these antibodies will enhance our ability to further characterize host-viral interactions, including specific roles played by viral proteins during infection, to acquire a better understanding of the pathophysiology of SARS-CoV-2 infections.

Original languageEnglish
Article number167583
JournalJournal of Molecular Biology
Volume434
Issue number10
DOIs
StatePublished - May 30 2022

Keywords

  • SARS-CoV-2
  • antibody
  • phage display

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