Development of low coherence light sheet illumination microscope for fluorescence-free bioimaging

Zhiguang Xu, Timothy E. Holy

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations


Light Sheet Illumination Microscopy (LSIM) is an imaging modality featuring the novel arrangement with the illumination axis perpendicular to the detection axis. In this technology a well defined light sheet is generated and aligned precisely to the focal plane of the microscope objective and thus only the thin in-focus layer of the sample is illuminated and imaged, thereby avoiding out-of-focus light. Besides the inherent optical sectioning function, other advantages include fast imaging speed, high longitudinal resolution and decreased light-induced damage. Though promising, this microscopy is currently restricted to imaging fluorescently labeled tissue; in inspection of intact tissue using scattered light, the acquired images suffer from intense speckles because of the severe coherence in the illumination. This work aims to build a microscope capable of achieving intrinsic images of the fluorescence-free sample with reduced or eliminated speckles, by developing a low coherence light sheet illumination. To diminish the spatial coherence, the sample is illuminated with tens of independent sub-beams (without inter-coherence) illuminating the FOV (Field Of View) of the microscope with diverse incident angles. The temporal coherence is dramatically reduced by employing a supercontinuum laser with a broad spectrum as the light source. The new microscopy significantly extends the functionality of Light Sheet Illumination Microscopy and will enable many new bioimaging applications.

Original languageEnglish
Title of host publicationNovel Optical Systems Design and Optimization XIV
StatePublished - Oct 28 2011
EventNovel Optical Systems Design and Optimization XIV - San Diego, CA, United States
Duration: Aug 22 2011Aug 22 2011

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X


ConferenceNovel Optical Systems Design and Optimization XIV
Country/TerritoryUnited States
CitySan Diego, CA


  • Bioimaging
  • Intrinsic imaging
  • Light sheet illumination microscopy
  • Low-coherence illumination
  • Speckle


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