Development of computational model for cell dose and DNA damage quantification of multicellular system

Ruirui Liu, Tianyu Zhao, Maciej H. Swat, Francisco J. Reynoso, Kathryn A. Higley

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Purpose: The aim of this study is to build a computational model to investigate the cell dose and cell DNA damage distribution of a multicellular tissue system under the irradiation. Materials and methods: In this work, we developed a computational model for quantifying cell dose and double strand break (DSB) number in a multicellular system by simulating the radiation transport in 2D and 3D cell culture. The model was based on an open-source radiation transport package, Geant4 with Geant4-DNA physics. First, the computational multicellular system was created using a developed program, CelllMaker. Second, the radiation transport simulation for cells was conducted using Geant4 package with the Geant4-DNA physics to obtain the cellular dose and cellular DSB yield. Results: Using the method described in this work, it is possible to obtain the cellular dose and DNA damage simultaneously. The developed model provides a solution for quantifying the cellular dose and cellular DNA damage which are not easily determined in a radiobiological experiment. Conclusions: With limited validation data for the model, this preliminary study provides a roadmap for building a comprehensive toolkit for simulating cellular dose and DNA damage of multicellular tissue systems.

Original languageEnglish
Pages (from-to)1484-1497
Number of pages14
JournalInternational Journal of Radiation Biology
Volume95
Issue number11
DOIs
StatePublished - Nov 2 2019

Keywords

  • DNA damage
  • Geant4
  • Geant4-DNA
  • Monte Carlo simulation
  • cellular dose
  • computational model
  • multicellular model

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