TY - JOUR
T1 - Development of Chemical and Metabolite Sensors for Rhodococcus opacus PD630
AU - DeLorenzo, Drew M.
AU - Henson, William R.
AU - Moon, Tae Seok
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/10/20
Y1 - 2017/10/20
N2 - Rhodococcus opacus PD630 is a nonmodel, Gram-positive bacterium that possesses desirable traits for biomass conversion, including consumption capabilities for lignocellulose-based sugars and toxic lignin-derived aromatic compounds, significant triacylglycerol accumulation, relatively rapid growth rate, and genetic tractability. However, few genetic elements have been directly characterized in R. opacus, limiting its application for lignocellulose bioconversion. Here, we report the characterization and development of genetic tools for tunable gene expression in R. opacus, including: (1) six fluorescent reporters for quantifying promoter output, (2) three chemically inducible promoters for variable gene expression, and (3) two classes of metabolite sensors derived from native R. opacus promoters that detect nitrogen levels or aromatic compounds. Using these tools, we also provide insights into native aromatic consumption pathways in R. opacus. Overall, this work expands the ability to control and characterize gene expression in R. opacus for future lignocellulose-based fuel and chemical production.
AB - Rhodococcus opacus PD630 is a nonmodel, Gram-positive bacterium that possesses desirable traits for biomass conversion, including consumption capabilities for lignocellulose-based sugars and toxic lignin-derived aromatic compounds, significant triacylglycerol accumulation, relatively rapid growth rate, and genetic tractability. However, few genetic elements have been directly characterized in R. opacus, limiting its application for lignocellulose bioconversion. Here, we report the characterization and development of genetic tools for tunable gene expression in R. opacus, including: (1) six fluorescent reporters for quantifying promoter output, (2) three chemically inducible promoters for variable gene expression, and (3) two classes of metabolite sensors derived from native R. opacus promoters that detect nitrogen levels or aromatic compounds. Using these tools, we also provide insights into native aromatic consumption pathways in R. opacus. Overall, this work expands the ability to control and characterize gene expression in R. opacus for future lignocellulose-based fuel and chemical production.
KW - Rhodococcus opacus
KW - aromatic
KW - lignin
KW - nitrogen
KW - promoter
KW - sensor
UR - http://www.scopus.com/inward/record.url?scp=85031928922&partnerID=8YFLogxK
U2 - 10.1021/acssynbio.7b00192
DO - 10.1021/acssynbio.7b00192
M3 - Article
C2 - 28745867
AN - SCOPUS:85031928922
SN - 2161-5063
VL - 6
SP - 1973
EP - 1978
JO - ACS synthetic biology
JF - ACS synthetic biology
IS - 10
ER -