Development of aptamer-based affinity assays using temperature gradient focusing: Minimization of the limit of detection

M. S. Munson; J. M. Meacham; D. Ross; L. E. Locascio

Development of aptamer-based affinity assays using temperature gradient focusing: Minimization of the limit of detection

M. S. Munson
, J. M. Meacham
, D. Ross
, L. E. Locascio

Research output: Contribution to conference › Paper › peer-review

Abstract

We demonstrate aptamer-based affinity assays using capillary electrophoresis (CE), temperature gradient focusing (TGF) and field amplified continuous sample injection TGF (FACSI-TGF). The optimal limit of detection (LOD) is determined for each platform, providing a basis for comparison. The LOD obtained with FACSI-TGF was lower than those obtained with CE, in spite of an inferior detector. We hypothesize that this is due to greater reproducibility of TGF and FACSI-TGF since they do not require sample injection. The lowest LOD achieved is more than an order of magnitude lower than a previously reported, CE-based assay for the same target. [1].

Original language	English
Pages	420-422
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

Aptamer
Capillary electrophoresis
Temperaure gradient focusing

Cite this

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title = "Development of aptamer-based affinity assays using temperature gradient focusing: Minimization of the limit of detection",

abstract = "We demonstrate aptamer-based affinity assays using capillary electrophoresis (CE), temperature gradient focusing (TGF) and field amplified continuous sample injection TGF (FACSI-TGF). The optimal limit of detection (LOD) is determined for each platform, providing a basis for comparison. The LOD obtained with FACSI-TGF was lower than those obtained with CE, in spite of an inferior detector. We hypothesize that this is due to greater reproducibility of TGF and FACSI-TGF since they do not require sample injection. The lowest LOD achieved is more than an order of magnitude lower than a previously reported, CE-based assay for the same target. [1].",

keywords = "Aptamer, Capillary electrophoresis, Temperaure gradient focusing",

author = "Munson, \{M. S.\} and Meacham, \{J. M.\} and D. Ross and Locascio, \{L. E.\}",

year = "2008",

language = "English",

pages = "420--422",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

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Development of aptamer-based affinity assays using temperature gradient focusing: Minimization of the limit of detection. / Munson, M. S.; Meacham, J. M.; Ross, D. et al.
2008. 420-422 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Development of aptamer-based affinity assays using temperature gradient focusing

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

AU - Munson, M. S.

AU - Meacham, J. M.

AU - Ross, D.

AU - Locascio, L. E.

PY - 2008

Y1 - 2008

N2 - We demonstrate aptamer-based affinity assays using capillary electrophoresis (CE), temperature gradient focusing (TGF) and field amplified continuous sample injection TGF (FACSI-TGF). The optimal limit of detection (LOD) is determined for each platform, providing a basis for comparison. The LOD obtained with FACSI-TGF was lower than those obtained with CE, in spite of an inferior detector. We hypothesize that this is due to greater reproducibility of TGF and FACSI-TGF since they do not require sample injection. The lowest LOD achieved is more than an order of magnitude lower than a previously reported, CE-based assay for the same target. [1].

AB - We demonstrate aptamer-based affinity assays using capillary electrophoresis (CE), temperature gradient focusing (TGF) and field amplified continuous sample injection TGF (FACSI-TGF). The optimal limit of detection (LOD) is determined for each platform, providing a basis for comparison. The LOD obtained with FACSI-TGF was lower than those obtained with CE, in spite of an inferior detector. We hypothesize that this is due to greater reproducibility of TGF and FACSI-TGF since they do not require sample injection. The lowest LOD achieved is more than an order of magnitude lower than a previously reported, CE-based assay for the same target. [1].

KW - Aptamer

KW - Capillary electrophoresis

KW - Temperaure gradient focusing

UR - https://www.scopus.com/pages/publications/84902524748

M3 - Paper

AN - SCOPUS:84902524748

SP - 420

EP - 422

Y2 - 12 October 2008 through 16 October 2008

ER -

Development of aptamer-based affinity assays using temperature gradient focusing: Minimization of the limit of detection

Abstract

Conference

Keywords

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