Abstract
We demonstrate aptamer-based affinity assays using capillary electrophoresis (CE), temperature gradient focusing (TGF) and field amplified continuous sample injection TGF (FACSI-TGF). The optimal limit of detection (LOD) is determined for each platform, providing a basis for comparison. The LOD obtained with FACSI-TGF was lower than those obtained with CE, in spite of an inferior detector. We hypothesize that this is due to greater reproducibility of TGF and FACSI-TGF since they do not require sample injection. The lowest LOD achieved is more than an order of magnitude lower than a previously reported, CE-based assay for the same target. [1].
Original language | English |
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Pages | 420-422 |
Number of pages | 3 |
State | Published - 2008 |
Event | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008 |
Conference
Conference | 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 |
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Country/Territory | United States |
City | San Diego, CA |
Period | 10/12/08 → 10/16/08 |
Keywords
- Aptamer
- Capillary electrophoresis
- Temperaure gradient focusing