Development of aptamer-based affinity assays using temperature gradient focusing: Minimization of the limit of detection

M. S. Munson, J. M. Meacham, D. Ross, L. E. Locascio

Research output: Contribution to conferencePaperpeer-review

Abstract

We demonstrate aptamer-based affinity assays using capillary electrophoresis (CE), temperature gradient focusing (TGF) and field amplified continuous sample injection TGF (FACSI-TGF). The optimal limit of detection (LOD) is determined for each platform, providing a basis for comparison. The LOD obtained with FACSI-TGF was lower than those obtained with CE, in spite of an inferior detector. We hypothesize that this is due to greater reproducibility of TGF and FACSI-TGF since they do not require sample injection. The lowest LOD achieved is more than an order of magnitude lower than a previously reported, CE-based assay for the same target. [1].

Original languageEnglish
Pages420-422
Number of pages3
StatePublished - 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Conference

Conference12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/TerritoryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

Keywords

  • Aptamer
  • Capillary electrophoresis
  • Temperaure gradient focusing

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