Development of an immunologically tolerated combination of fluorescent proteins for in vivo two-photon imaging

Selamawit Gossa, Debasis Nayak, Bernd H. Zinselmeyer, Dorian B. McGavern

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Combinations of fluorescent proteins (FPs) are routinely used for multi-parameter in vivoimaging experiments to visualize tagged proteins or cell populations of interest. Studies involving FPs are often limited by spectral overlap, toxicity, relative quantum efficiency, and the potential for immunological rejection upon transfer into a non-tolerant recipient. Here we evaluate the immunologic visibility of several commonly used FPs by the murine immune system and identify a spectrally compatible, immunologically tolerated combination of FPs well suited for in vivotwo-photon imaging.

Original languageEnglish
Article number6664
JournalScientific reports
Volume4
DOIs
StatePublished - Oct 17 2014

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