@article{9541287054dd410e88d71e60b4c198b1,
title = "Development of a Positron Emission Tomography Radiotracer for Imaging Elevated Levels of Superoxide in Neuroinflammation",
abstract = " Reactive oxygen species (ROS) are believed to play a major role in the proinflammatory, M1-polarized form of neuroinflammation. However, it has been difficult to assess the role of ROS and their role in neuroinflammation in animal models of disease because of the absence of probes capable of measuring their presence with the functional imaging technique positron emission tomography (PET). This study describes the synthesis and in vivo evaluation of [ 18 F]ROStrace, a radiotracer for imaging superoxide in vivo with PET, in an LPS model of neuroinflammation. [ 18 F]ROStrace was found to rapidly cross the blood-brain barrier (BBB) and was trapped in the brain of LPS-treated animals but not the control group. [ 18 F]ox-ROStrace, the oxidized form of [ 18 F]ROStrace, did not cross the BBB. These data suggest that [ 18 F]ROStrace is a suitable radiotracer for imaging superoxide levels in the central nervous system with PET.",
author = "Catherine Hou and Hsieh, {Chia Ju} and Shihong Li and Hsiaoju Lee and Graham, {Thomas J.} and Kuiying Xu and Weng, {Chi Chang} and Doot, {Robert K.} and Wenhua Chu and Chakraborty, {Subhasish K.} and Dugan, {Laura L.} and Mintun, {Mark A.} and Mach, {Robert H.}",
note = "Funding Information: This research was funded by a grant from the Michael J. Fox Foundation (R.H.M.) and National Institutes of Health Grant AG033679-05 (L.L.D.). Funding Information: R.H.M., L.L.D., and M.A.M. conceived of the project. C.H. and R.H.M. managed the study. T.J.G., K.X., and W.C. conducted organic synthesis. S.L. and T.J.G. conducted radiochemistry. S.L. and H.L. performed analytical quality control. S.K.C. and L.L.D. conducted the in vitro ROS assay. C.H. prepared the animals and performed the imaging studies. C.-C.W. performed the autoradiography. C.-J.H. and R.K.D. processed images and performed the PET data analysis. C.H., C.-J.H., S.L., K.X., and R.H.M. wrote the manuscript. Funding This research was funded by a grant from the Michael J. Fox Foundation (R.H.M.) and National Institutes of Health Grant AG033679-05 (L.L.D.). Notes The authors declare the following competing financial interest(s): The radiotracer described in this work is covered under U.S. Patent 9,035,057 B2 (W.C., L.L.D., M.A.M., and R.H.M., inventors). Publisher Copyright: {\textcopyright} 2017 American Chemical Society.",
year = "2018",
month = mar,
day = "21",
doi = "10.1021/acschemneuro.7b00385",
language = "English",
volume = "9",
pages = "578--586",
journal = "ACS Chemical Neuroscience",
issn = "1948-7193",
number = "3",
}