Development and standardization of a rapid, PCR-based method for the detection of Wuchereria bancrofti in mosquitoes, for xenomonitoring the human prevalence of bancroftian filariasis.

S. A. Williams, S. J. Laney, L. A. Bierwert, L. J. Saunders, D. A. Boakye, P. Fischer, D. Goodman, H. Helmy, S. L. Hoti, V. Vasuki, P. J. Lammie, C. Plichart, R. M. Ramzy, E. A. Ottesen

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58 Scopus citations

Abstract

PCR has recently been studied as a promising tool for monitoring the progress of efforts to eliminate lymphatic filariasis. PCR can be used to test concurrently at least 30 pools, with as many as 40 mosquitoes in each pool, for the presence of filarial larvae. The SspI PCR assay for the detection of Wuchereria bancrofti DNA in pools of mosquitoes has been used since 1994 in a variety of laboratories worldwide. During that time, the original assay has been modified in these different laboratories and no standardized assay currently exists. In an effort to standardize and improve the assay, a meeting was held on 15-16 November 2001, at Emory University in Atlanta, with representatives from most of the laboratories currently using the assay. The first round of testing was designed to test the four most promising methods for DNA extraction from pools of mosquitoes. Two of the four methods stood out as clearly the best and these will be now optimised and evaluated in two further rounds of testing.

Original languageEnglish
Pages (from-to)S41-46
JournalAnnals of tropical medicine and parasitology
Volume96 Suppl 2
StatePublished - Dec 2002
Externally publishedYes

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    Williams, S. A., Laney, S. J., Bierwert, L. A., Saunders, L. J., Boakye, D. A., Fischer, P., Goodman, D., Helmy, H., Hoti, S. L., Vasuki, V., Lammie, P. J., Plichart, C., Ramzy, R. M., & Ottesen, E. A. (2002). Development and standardization of a rapid, PCR-based method for the detection of Wuchereria bancrofti in mosquitoes, for xenomonitoring the human prevalence of bancroftian filariasis. Annals of tropical medicine and parasitology, 96 Suppl 2, S41-46.