Development and evaluation of a novel, semiautomated clostridium difficile typing platform

Lars F. Westblade; Robin R. Chamberland; Duncan MacCannell; Rachel Collins; Erik R. Dubberke; W. Michael Dunne; Carey Ann D. Burnham

doi:10.1128/JCM.02627-12

Development and evaluation of a novel, semiautomated clostridium difficile typing platform

Lars F. Westblade
, Robin R. Chamberland
, Duncan MacCannell
, Rachel Collins
, Erik R. Dubberke
, W. Michael Dunne
, Carey Ann D. Burnham

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We describe a novel, semiautomated Clostridium difficile typing platform that is based on PCR-ribotyping in conjunction with a semiautomated molecular typing system. The platform is reproducible with minimal intra- or interassay variability. This method exhibited a discriminatory index of 0.954 and is therefore comparable to more arduous typing systems, such as pulsedfield gel electrophoresis.

Original language	English
Pages (from-to)	621-624
Number of pages	4
Journal	Journal of clinical microbiology
Volume	51
Issue number	2
DOIs	https://doi.org/10.1128/JCM.02627-12
State	Published - Feb 2013

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abstract = "We describe a novel, semiautomated Clostridium difficile typing platform that is based on PCR-ribotyping in conjunction with a semiautomated molecular typing system. The platform is reproducible with minimal intra- or interassay variability. This method exhibited a discriminatory index of 0.954 and is therefore comparable to more arduous typing systems, such as pulsedfield gel electrophoresis.",

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AU - Dunne, W. Michael

AU - Burnham, Carey Ann D.

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AB - We describe a novel, semiautomated Clostridium difficile typing platform that is based on PCR-ribotyping in conjunction with a semiautomated molecular typing system. The platform is reproducible with minimal intra- or interassay variability. This method exhibited a discriminatory index of 0.954 and is therefore comparable to more arduous typing systems, such as pulsedfield gel electrophoresis.

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Development and evaluation of a novel, semiautomated clostridium difficile typing platform

Abstract

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