Development and evaluation of a novel, semiautomated clostridium difficile typing platform

Lars F. Westblade, Robin R. Chamberland, Duncan MacCannell, Rachel Collins, Erik R. Dubberke, W. Michael Dunne, Carey Ann D. Burnham

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

We describe a novel, semiautomated Clostridium difficile typing platform that is based on PCR-ribotyping in conjunction with a semiautomated molecular typing system. The platform is reproducible with minimal intra- or interassay variability. This method exhibited a discriminatory index of 0.954 and is therefore comparable to more arduous typing systems, such as pulsedfield gel electrophoresis.

Original languageEnglish
Pages (from-to)621-624
Number of pages4
JournalJournal of clinical microbiology
Volume51
Issue number2
DOIs
StatePublished - Feb 2013

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