TY - GEN
T1 - Development and characterization of nanoparticles as imaging probes for correlative optical and electron microscopy
AU - Thapa-Chetri, Prem S.
AU - Santiago-Robles, Carolina M.
AU - Martinez-Rivera, Noraida
AU - Torres-Vazquez, Irma I.
AU - Joshi, Vishwas N.
AU - Powell, Richard D.
AU - Rosa-Molinar, Eduardo
N1 - Publisher Copyright:
© 2017 IEEE.
PY - 2017/11/21
Y1 - 2017/11/21
N2 - Nanoparticles of several different compositions and lattice structures that have been characterized by transmission electron microscopy, scanning/transmission electron microscopy, X-ray spectroscopic (EDX) analysis, and diffraction patterns may be conjugated to biological targeting agents for use as labels that can localize and differentiate multiple targets at nanometer resolution in complex biological specimens. Small (most 0.8-3.0 nm) particles composed of iron oxide/carbon, gold, iridium and bismuth/silica may be differentiated by both EDX and diffraction patterns and offer the following advantages over conventional colloidal gold labeling: smaller probe size and faster specimen penetration, higher labeling precision, higher, more consistent labeling density, and nearer quantitative labeling. The combination of small probe size and number of compositions available affords increased multiplexing capability.
AB - Nanoparticles of several different compositions and lattice structures that have been characterized by transmission electron microscopy, scanning/transmission electron microscopy, X-ray spectroscopic (EDX) analysis, and diffraction patterns may be conjugated to biological targeting agents for use as labels that can localize and differentiate multiple targets at nanometer resolution in complex biological specimens. Small (most 0.8-3.0 nm) particles composed of iron oxide/carbon, gold, iridium and bismuth/silica may be differentiated by both EDX and diffraction patterns and offer the following advantages over conventional colloidal gold labeling: smaller probe size and faster specimen penetration, higher labeling precision, higher, more consistent labeling density, and nearer quantitative labeling. The combination of small probe size and number of compositions available affords increased multiplexing capability.
UR - http://www.scopus.com/inward/record.url?scp=85041186737&partnerID=8YFLogxK
U2 - 10.1109/NANO.2017.8117452
DO - 10.1109/NANO.2017.8117452
M3 - Conference contribution
AN - SCOPUS:85041186737
T3 - 2017 IEEE 17th International Conference on Nanotechnology, NANO 2017
SP - 159
EP - 163
BT - 2017 IEEE 17th International Conference on Nanotechnology, NANO 2017
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 17th IEEE International Conference on Nanotechnology, NANO 2017
Y2 - 25 July 2017 through 28 July 2017
ER -