TY - JOUR
T1 - Development and characterization of microsatellite loci for Tabebuia cassinoides (Bignoniaceae)
AU - Pretti, V. Q.
AU - Françoso, E.
AU - Sforça, D. A.
AU - Pinheiro, F.
AU - Meyer, D.
AU - Lohmann, L. G.
PY - 2014/7/25
Y1 - 2014/7/25
N2 - Tabebuia cassinoides (Lam.) DC., popularly known as caxeta, is a tree species that belongs to the plant family Bignoniaceae. This species is endemic to the Brazilian Atlantic Forest and is widely exploited commercially. To date, little is known about its genetic structure, preventing the establishment of adequate management plans for this taxon. The objective of this study was to construct a microsatellite-enriched genomic library for T. cassinoides to select polymorphic loci, and standardize polymerase chain reaction amplification conditions. Of the 15 loci examined, 5 were polymorphic. The number of alleles per locus ranged from 2 to 8, with a mean of 4.4. The microsatellite loci described here represent the basis for detailed population genetic studies of this species, which will greatly contribute for the development of better conservation strategies for this taxon.
AB - Tabebuia cassinoides (Lam.) DC., popularly known as caxeta, is a tree species that belongs to the plant family Bignoniaceae. This species is endemic to the Brazilian Atlantic Forest and is widely exploited commercially. To date, little is known about its genetic structure, preventing the establishment of adequate management plans for this taxon. The objective of this study was to construct a microsatellite-enriched genomic library for T. cassinoides to select polymorphic loci, and standardize polymerase chain reaction amplification conditions. Of the 15 loci examined, 5 were polymorphic. The number of alleles per locus ranged from 2 to 8, with a mean of 4.4. The microsatellite loci described here represent the basis for detailed population genetic studies of this species, which will greatly contribute for the development of better conservation strategies for this taxon.
KW - Bignoniaceae
KW - Brazilian atlantic forest
KW - Microsatellite primers
KW - Tabebuia cassinoides
UR - https://www.scopus.com/pages/publications/84904979081
U2 - 10.4238/2014.July.25.15
DO - 10.4238/2014.July.25.15
M3 - Article
C2 - 25117317
AN - SCOPUS:84904979081
SN - 1676-5680
VL - 13
SP - 5601
EP - 5605
JO - Genetics and Molecular Research
JF - Genetics and Molecular Research
IS - 3
ER -