Development and Characterization of a Chemically Defined Food for Drosophila

Wen Chih Lee, Craig A. Micchelli

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Diet can affect a spectrum of biological processes ranging from behavior to cellular metabolism. Yet, the precise role of an individual dietary constituent can be a difficult variable to isolate experimentally. A chemically defined food (CDF) permits the systematic evaluation of individual macro- and micronutrients. In addition, CDF facilitates the direct comparison of data obtained independently from different laboratories. Here, we report the development and characterization of a CDF for Drosophila. We show that CDF can support the long-term culture of laboratory strains and demonstrate that this formulation has utility in isolating macronutrient from caloric density requirements in studies of development, longevity and reproduction.

Original languageEnglish
Article numbere67308
JournalPloS one
Volume8
Issue number7
DOIs
StatePublished - Jul 2 2013

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