Toxoplasma gondii undergoes its sexual phase within the feline intestine. The sexual stages have been an important tool for understanding the biology of the parasite using forward genetics approaches. Genetic crosses were performed by feeding cats bradyzoites from T. gondii strains that differ in virulence and drug resistance. Using progeny from these crosses, the genes responsible for virulence and other biologically important phenotypes have been mapped using classical forward genetic techniques. This chapter reviews the principles of forward genetics in T. gondii and illustrates how the classical genetics approach enabled identification of Toxoplasma virulence genes including the ROP family of rhoptry kinases and pseudokinases.
|Title of host publication||Toxoplasma Gondii|
|Subtitle of host publication||The Model Apicomplexan - Perspectives and Methods: Second Edition|
|Number of pages||26|
|State||Published - Sep 1 2013|
- Drug resistance
- Genetic cross
- Rhoptry kinase