Development and Application of Classical Genetics in Toxoplasma gondii

James W. Ajioka, L. David Sibley

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Toxoplasma gondii undergoes its sexual phase within the feline intestine. The sexual stages have been an important tool for understanding the biology of the parasite using forward genetics approaches. Genetic crosses were performed by feeding cats bradyzoites from T. gondii strains that differ in virulence and drug resistance. Using progeny from these crosses, the genes responsible for virulence and other biologically important phenotypes have been mapped using classical forward genetic techniques. This chapter reviews the principles of forward genetics in T. gondii and illustrates how the classical genetics approach enabled identification of Toxoplasma virulence genes including the ROP family of rhoptry kinases and pseudokinases.

Original languageEnglish
Title of host publicationToxoplasma Gondii
Subtitle of host publicationThe Model Apicomplexan - Perspectives and Methods: Second Edition
PublisherElsevier Ltd
Pages551-576
Number of pages26
ISBN (Print)9780123964816
DOIs
StatePublished - Sep 1 2013

Keywords

  • Drug resistance
  • Genetic cross
  • Genetics
  • Rhoptry kinase
  • Virulence

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    Ajioka, J. W., & Sibley, L. D. (2013). Development and Application of Classical Genetics in Toxoplasma gondii. In Toxoplasma Gondii: The Model Apicomplexan - Perspectives and Methods: Second Edition (pp. 551-576). Elsevier Ltd. https://doi.org/10.1016/B978-0-12-396481-6.00016-7