Development and Application of Classical Genetics in Toxoplasma gondii

James W. Ajioka; L. David Sibley

doi:10.1016/B978-0-12-396481-6.00016-7

Development and Application of Classical Genetics in Toxoplasma gondii

James W. Ajioka, L. David Sibley

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Scopus citations

Abstract

Toxoplasma gondii undergoes its sexual phase within the feline intestine. The sexual stages have been an important tool for understanding the biology of the parasite using forward genetics approaches. Genetic crosses were performed by feeding cats bradyzoites from T. gondii strains that differ in virulence and drug resistance. Using progeny from these crosses, the genes responsible for virulence and other biologically important phenotypes have been mapped using classical forward genetic techniques. This chapter reviews the principles of forward genetics in T. gondii and illustrates how the classical genetics approach enabled identification of Toxoplasma virulence genes including the ROP family of rhoptry kinases and pseudokinases.

Original language	English
Title of host publication	Toxoplasma Gondii
Subtitle of host publication	The Model Apicomplexan - Perspectives and Methods: Second Edition
Publisher	Elsevier Ltd
Pages	551-576
Number of pages	26
ISBN (Print)	9780123964816
DOIs	https://doi.org/10.1016/B978-0-12-396481-6.00016-7
State	Published - Sep 2013

Keywords

Drug resistance
Genetic cross
Genetics
Rhoptry kinase
Virulence

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10.1016/B978-0-12-396481-6.00016-7

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title = "Development and Application of Classical Genetics in Toxoplasma gondii",

abstract = "Toxoplasma gondii undergoes its sexual phase within the feline intestine. The sexual stages have been an important tool for understanding the biology of the parasite using forward genetics approaches. Genetic crosses were performed by feeding cats bradyzoites from T. gondii strains that differ in virulence and drug resistance. Using progeny from these crosses, the genes responsible for virulence and other biologically important phenotypes have been mapped using classical forward genetic techniques. This chapter reviews the principles of forward genetics in T. gondii and illustrates how the classical genetics approach enabled identification of Toxoplasma virulence genes including the ROP family of rhoptry kinases and pseudokinases.",

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AU - Sibley, L. David

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AB - Toxoplasma gondii undergoes its sexual phase within the feline intestine. The sexual stages have been an important tool for understanding the biology of the parasite using forward genetics approaches. Genetic crosses were performed by feeding cats bradyzoites from T. gondii strains that differ in virulence and drug resistance. Using progeny from these crosses, the genes responsible for virulence and other biologically important phenotypes have been mapped using classical forward genetic techniques. This chapter reviews the principles of forward genetics in T. gondii and illustrates how the classical genetics approach enabled identification of Toxoplasma virulence genes including the ROP family of rhoptry kinases and pseudokinases.

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Development and Application of Classical Genetics in Toxoplasma gondii

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Keywords

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